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The recombination landscape and multiple QTL mapping in a Solanum tuberosum cv. ‘Atlantic’-derived F 1 population
Heredity ( IF 3.8 ) Pub Date : 2021-03-22 , DOI: 10.1038/s41437-021-00416-x
Guilherme da Silva Pereira 1, 2, 3 , Marcelo Mollinari 1, 2 , Mitchell J Schumann 1, 4 , Mark E Clough 1, 5 , Zhao-Bang Zeng 1, 2 , G Craig Yencho 1
Affiliation  

There are many challenges involved with the genetic analyses of autopolyploid species, such as the tetraploid potato, Solanum tuberosum (2n = 4x = 48). The development of new analytical methods has made it valuable to re-analyze an F1 population (n = 156) derived from a cross involving ‘Atlantic’, a widely grown chipping variety in the USA. A fully integrated genetic map with 4285 single nucleotide polymorphisms, spanning 1630 cM, was constructed with MAPpoly software. We observed that bivalent configurations were the most abundant ones (51.0~72.4% depending on parent and linkage group), though multivalent configurations were also observed (2.2~39.2%). Seven traits were evaluated over four years (2006–8 and 2014) and quantitative trait loci (QTL) mapping was carried out using QTLpoly software. Based on a multiple-QTL model approach, we detected 21 QTL for 15 out of 27 trait-year combination phenotypes. A hotspot on linkage group 5 was identified with co-located QTL for maturity, plant yield, specific gravity, and internal heat necrosis resistance evaluated over different years. Additional QTL for specific gravity and dry matter were detected with maturity-corrected phenotypes. Among the genes around QTL peaks, we found those on chromosome 5 that have been previously implicated in maturity (StCDF1) and tuber formation (POTH1). These analyses have the potential to provide insights into the biology and breeding of tetraploid potato and other autopolyploid species.



中文翻译:

马铃薯 cv. 中的重组景观和多个 QTL 映射。“大西洋”派生的 F 1 种群

同源多倍体物种的遗传分析涉及许多挑战,例如四倍体马铃薯Solanum tuberosum (2 n  = 4 x  = 48)。新分析方法的发展使得重新分析 F 1群体(n = 156) 源自与“Atlantic”杂交,这是一种在美国广泛种植的切片品种。使用 MAPpoly 软件构建了具有 4285 个单核苷酸多态性、跨越 1630 cM 的完全整合的遗传图谱。我们观察到二价构型是最丰富的(51.0~72.4%,取决于亲本和连锁群),但也观察到多价构型(2.2~39.2%)。在四年(2006-8 年和 2014 年)期间对七个性状进行了评估,并使用 QTLpoly 软件进行了数量性状位点 (QTL) 定位。基于多 QTL 模型方法,我们检测到 27 个性状年组合表型中的 15 个有 21 个 QTL。连锁群 5 上的一个热点被确定为具有同位 QTL,用于评估不同年份的成熟度、植物产量、比重和内部热坏死抗性。使用成熟度校正表型检测到比重和干物质的额外 QTL。在 QTL 峰附近的基因中,我们发现 5 号染色体上的那些基因以前与成熟有关(StCDF1)和块茎形成(POTH1)。这些分析有可能深入了解四倍体马铃薯和其他同源多倍体物种的生物学和育种。

更新日期:2021-03-23
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