The article reviews applications of flow cytometry sorting in manufacturing of pharmaceuticals. Flow cytometry sorting is an extremely powerful tool for monitoring, screening and separating single cells based on any property that can be measured by flow cytometry. Different applications of flow cytometry sorting are classified into groups and discussed in separate sections as follows: (a) isolation of cell types, (b) high throughput screening, (c) cell surface display, (d) droplet fluorescent-activated cell sorting (FACS). Future opportunities are identified including: (a) sorting of particular fractions of the cell population based on a property of interest for generating inoculum that will result in improved outcomes of cell cultures and (b) the use of population balance models in combination with FACS to design and optimize cell cultures.

中文翻译：

---

流式细胞仪分选在制药行业的应用：综述

本文回顾了流式细胞仪分选在药品生产中的应用。流式细胞仪分选是一种非常强大的工具，可根据可通过流式细胞仪测量的任何特性监测、筛选和分离单个细胞。流式细胞仪分选的不同应用分为几组，并在单独的部分中讨论如下：（a）细胞类型的分离，（b）高通量筛选，（c）细胞表面展示，（d）液滴荧光激活细胞分选（流式细胞仪）。确定了未来的机会，包括：(a) 根据感兴趣的特性对细胞群的特定部分进行分类，以产生将改善细胞培养结果的接种物，以及 (b) 使用与 FACS 相结合的群体平衡模型设计和优化细胞培养。