The cytochrome P450 2E1 (CYP2E1) enzyme encoded by CYP2E1 plays an important role in the metabolism of organic compounds in mammalian liver cells. In this study, the plasmid pCAMBIA2300 harboring the rabbit CYP2E1 was transferred into Agrobacterium tumefaciens; this was then transferred into internodes of Ardisia pusilla. Shoot primordia, whose appearance resembles that of the in vitro protocorm-like body of orchids, regenerated from 121 of 332 internodes that were cultured in selection medium supplemented with 5 mg·L⁻¹ kanamycin. PCR analysis confirmed that 25 of 89 putative transgenic plants (28.0%) stably harbored the CYP2E1 and NPTII genes, and Southern blot analysis revealed the presence of one to three copies of CYP2E1 within the genome of the 25 transgenic plants. The mRNA expression of the transgene was confirmed in 12 of 25 CYP2E1-transgenic plants by Northern blot analysis. Western blot analysis indicated the presence of a 53-kDa recombinant rabbit CYP2E1 protein in the 12 CYP2E1-transgenic A. pusilla plants. The aniline 4-hydroxylase activity of rabbit CYP2E1 in nine CYP2E1-transgenic A. pusilla plants was higher than that in non-transgenic A. pusilla plants. There was a statistically significant increase (5.1–6.1-fold) in the toluene removal ability of the transgenic line CYP2E1-2–2 compared to that observed in wild-type Ardisia plants at different times after exogenous toluene exposure.

细胞色素P450 2E1（CYP2E1）由编码的酶CYP2E1起着在哺乳动物肝细胞有机化合物的代谢中起重要作用。在这项研究中，将携带兔CYP2E1的质粒pCAMBIA2300转移到根癌农杆菌中。然后转移到Ardisia pusilla的节间。茎原基的外观类似于兰花的体外原球茎状体，是从在补充有5 mg·L ^-1卡那霉素的选择培养基中培养的332个节间中的121个中再生而来的。PCR分析证实89种推定的转基因植物中有25种（28.0％）稳定地包含CYP2E1和NPTII基因，Southern印迹分析显示，在25个转基因植物的基因组中存在1-3个CYP2E1拷贝。通过Northern印迹分析在25株CYP2E1转基因植物中的12株中确认了转基因的mRNA表达。Western印迹分析表明在12个CYP2E1转基因的A. pusilla植物中存在53 kDa重组兔CYP2E1蛋白。九种CYP2E1转基因A. pusilla植物中兔CYP2E1的苯胺4-羟化酶活性高于非转基因A. pusilla中的动物。植物。与野生型Ardisia植物在外源甲苯暴露后的不同时间观察到的结果相比，转基因品系CYP2E1-2-2的甲苯去除能力有统计学上的显着提高（5.1-6.1倍）。