Ginkgo biloba flavonoids are important natural bioactive compounds with strong physiological effects. To develop an eco‐friendly and effective method for extracting flavonoids from plants, a microbial method involving lignocellulose‐degrading bacterial enzymes was developed to improve flavonoid extraction from Ginkgo biloba leaves. In this study, a newly isolated bacterial strain, Paenarthrobacter sp. S1.3, was employed for the fermentation processing of Ginkgo biloba leaf powder. The enzymatic characteristics of xylanase, CMCase, polygalacturonase, and β‐glucosidase from Paenarthrobacter sp. S1.3 were also investigated. The major fermentation parameters for the microbial extraction of Ginkgo biloba flavonoids were optimized using response surface methodology. Under optimal conditions (30.6 °C, initial pH 7.6, and fermentation time 25 h), a total flavonoid yield of 34.15 ± 0.52 mg g⁻¹ dry weight was obtained, which was 2.11 fold and 1.42 fold higher than the yields obtained from the unfermented and ethanol extraction methods, respectively. The optimized results also suggest that the polygalacturonase and β‐glucosidase might play an important role in flavonoid extraction. Fermentation utilizing lignocellulose‐degrading bacteria was a feasible green approach to improve the extraction of natural compounds from plants. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译：

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使用木质纤维素分解细菌Paenarthrobacter sp。从银杏中提取黄酮。并通过响应面方法进行了优化

银杏叶黄酮是重要的天然生物活性化合物，具有很强的生理作用。为了开发一种从植物中提取黄酮类化合物的环保，有效的方法，开发了一种涉及降解木质纤维素的细菌酶的微生物方法，以改善银杏叶中黄酮类化合物的提取。在这项研究中，一个新分离的细菌菌株，Paenarthrobacter sp。S1.3，用于银杏叶粉的发酵加工。潘氏杆菌属的木聚糖酶，CMCase，聚半乳糖醛酸酶和β-葡萄糖苷酶的酶学特征。还对S1.3进行了调查。微生物提取of的主要发酵参数。使用响应面方法对银杏叶黄酮进行了优化。在最佳条件下（30.6°C，初始pH 7.6，发酵时间25 h），获得的总类黄酮产量为34.15±0.52 mg g ^-1干重，比从中得到的产量高2.11倍和1.42倍未发酵和乙醇提取方法分别。优化结果还表明，聚半乳糖醛酸酶和β-葡萄糖苷酶可能在类黄酮提取中起重要作用。利用降解木质纤维素的细菌进行发酵是一种可行的绿色方法，可以改善植物中天然化合物的提取。©2021化学工业协会和John Wiley＆Sons，Ltd