Abstract

Chinese geese are domesticated from wild swan (Anser cygnoides), which have maintained a strong capacity of fat deposit. Fat mainly distributes subcutaneous, abdominal, intermuscular or intramuscular in poultry, and they display some special physiological and biochemical characteristics in different parts. This study aimed to characterize the adipogenesis in intramuscular (IM) and subcutaneous (SC) adipocytes of the goose. Here, IM and SC preadipocytes were isolated from the 26-day-old Yangzhou goose embryos, and subsequently induced them to differentiate into mature adipocytes. The results showed that SC preadipocytes grew a little faster than IM preadipocytes during the logarithmic multiplication phase (p < 0.05). Meanwhile, SC adipocytes accumulated more lipid than IM adipocytes during the differentiation process in vitro (p < 0.01). In addition, the expression level of key genes involved in adipogenesis, including peroxisome proliferator activated receptor γ/α (PPARγ/α), CCAAT/enhancer binding protein-α/β (C/EBPα/β), adipocyte fatty acid binding protein 4 (FABP4), and lipoprotein lipase (LPL) were detected. PPARγ, C/EBPα, FABP4, and LPL, were predominantly expressed in SC adipocytes, whereas C/EBPβ was highly expressed in IM adipocytes. Taken together, these results demonstrated that SC preadipocytes tended to grow faster and accumulate more lipid than IM adipocytes, and show greater potential for adipogenesis.

摘要

大雁由野天鹅（Anser cygnoides）驯化而来，具有很强的脂肪储存能力。家禽脂肪主要分布于皮下、腹部、肌肉间或肌肉内，在不同部位表现出一些特殊的生理生化特性。本研究旨在表征鹅的肌内 (IM) 和皮下 (SC) 脂肪细胞的脂肪生成。在这里，从 26 天大的扬州鹅胚胎中分离出 IM 和 SC 前脂肪细胞，随后诱导它们分化为成熟脂肪细胞。结果显示，在对数倍增期，SC 前脂肪细胞的生长速度略快于 IM 前脂肪细胞（p < 0.05)。同时，在体外分化过程中，SC 脂肪细胞比 IM 脂肪细胞积累了更多的脂质( p  < 0.01)。此外，参与脂肪形成的关键基因的表达水平，包括过氧化物酶体增殖物激活受体γ/α（PPARγ/α）、CCAAT/增强子结合蛋白-α/β（C/EBPα/β）、脂肪细胞脂肪酸结合蛋白4 ( FABP4 ) 和脂蛋白脂肪酶 ( LPL ) 被检测到。PPARγ、C/EBPα、FABP4和LPL主要在 SC 脂肪细胞中表达，而C/EBPβ在 IM 脂肪细胞中高表达。总之，这些结果表明 SC 前脂肪细胞往往比 IM 脂肪细胞生长得更快并积累更多的脂质，并且显示出更大的脂肪生成潜力。