Background and Aim. A wet suction technique (“wet” technique) has been developed to improve the quality of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) for sampling various solid lesions. However, no studies have reported on the wet technique for EUS-FNA for gastrointestinal (GI) subepithelial lesions (SELs). We conducted a pilot randomized crossover trial to explore whether the wet technique could be useful with regard to tissue adequacy of upper GI-SELs (UGI-SELs) compared to the conventional EUS-FNA technique (“dry” technique). Methods. Twenty-six patients with UGI-SELs indicated for EUS-FNA were randomly assigned to the dry-first arm using the dry technique for the first two passes or the wet-first arm using the wet technique for the first two passes using a cross-over design with a ratio of 1 : 1. The primary endpoint was the cellularity score of the EUS-FNA specimens rated on a 4-point scale (0-3). The secondary endpoints were the factors influencing cellularity in each suction technique. Results. The mean cellularity score was for the wet technique and for the dry technique (). Logistic regression analysis showed that higher cellularity may be related to the final diagnosis of gastrointestinal stromal tumors in the dry technique and the SEL location in the upper stomach in the wet technique. Conclusion. The wet EUS-FNA technique failed to show a potential for improved cellularity of specimens compared to the dry technique for UGI-SELs.

中文翻译：

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上消化道上皮下病变湿吸法和超声内镜引导细针抽吸常规技术的随机交叉试验

背景和目的。已开发出一种湿抽吸技术（“湿”技术）以提高内镜超声引导下细针抽吸 (EUS-FNA) 对各种实体病灶进行取样的质量。然而，尚无关于 EUS-FNA 用于胃肠道 (GI) 上皮下病变 (SELs) 的湿法技术的研究报告。我们进行了一项试点随机交叉试验，以探索与传统的 EUS-FNA 技术（“干”技术）相比，湿技术在上 GI-SEL (UGI-SEL) 的组织充足性方面是否有用。方法. 26 名有 UGI-SEL 的患者被随机分配到前两次通过使用干技术进行 EUS-FNA 的先干治疗组或使用湿技术使用交叉技术进行前两次通过的湿先治疗组。以 1 : 1 的比例过度设计。主要终点是 EUS-FNA 标本的细胞结构评分，采用 4 分制 (0-3)。次要终点是影响每种抽吸技术中细胞结构的因素。结果。平均细胞性评分为对于湿法和对于干法（）。Logistic回归分析表明，较高的细胞数量可能与干法中胃肠道间质瘤的最终诊断和湿法中上胃的SEL位置有关。结论。与用于 UGI-SEL 的干技术相比，湿 EUS-FNA 技术未能显示出改善标本细胞结构的潜力。