Protomyces is an understudied genus of yeast-like fungi currently defined as phytopathogens of only Umbelliferae and Compositae. Species relationships and boundaries remain controversial and molecular data are lacking. Of the 82 named Protomyces, we found few recent studies and six available cultures. We previously isolated Protomyces strains from wild Arabidopsis thaliana, a member of Brassicaceae, a family distant from accepted Protomyces hosts. We previously sequenced the genomes of all available Protomyces species, and P. arabidopsidicola sp. nov. strain C29, from Arabidopsis. Phylogenomics suggests this new species occupied a unique position in the genus. Genomic, morphological, and physiological characteristics distinguished P. arabidopsidicola sp. nov. from other Protomyces. Nuclear gene phylogenetic marker analysis suggests actin1 gene DNA sequences could be used with nuclear ribosomal DNA internal transcribed spacer sequences for rapid identification of Protomyces species. Previous studies demonstrated P. arabidopsidicola sp. nov. could persist on the Arabidopsis phyllosphere and Protomyces sequences were discovered on Arabidopsis at multiple sites in different countries. We conclude that the strain C29 represents a novel Protomyces species and propose the name of P. arabidopsidicola sp. nov. Consequently, we propose that Protomyces is not strictly associated only with the previously recognized host plants.

中文翻译：

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一种新颖的拟南芥叶围居民Protomyces物种和属的复检Protomyces基于基因组序列数据

Protomyces是一种酵母菌类真菌，尚未被广泛研究，目前仅被定义为伞形科和菊科的植物病原体。物种之间的关系和边界仍然存在争议，并且缺乏分子数据。在这82个命名为Protomyces的物种中，我们发现最近的研究很少，只有六种可用的文化。我们以前从野生拟南芥（芸苔科的成员）中分离出了原生藻菌株，该家族与公认的原生藻寄主相距甚远。我们之前对所有可用的原生藻和P. arabidopsidicola sp。的基因组进行了测序。十一月 来自拟南芥的菌株C29。生理学研究表明，该新物种在该属中占有独特的位置。基因组学，形态学和生理学特征使拟南芥（P. arabidopsidicola sp。）十一月 从其他Protomyces。核基因系统发生标记分析表明，actin1基因DNA序列可与核糖体DNA内部转录的间隔区序列一起使用，以快速鉴定原形藻的种类。先前的研究表明拟南芥体育。十一月 在拟南芥的叶球上可以持续存在，并且在不同国家的多个地点的拟南芥上发现了Protomyces序列。我们得出的结论是，菌株C29代表了一个新的Protomyces物种，并提出了P. arabidopsidicola sp。的名称。十一月 因此，我们建议原生质体不仅仅与先前公认的寄主植物严格相关。在拟南芥的叶球上可以持续存在，并且在不同国家的多个地点的拟南芥上发现了Protomyces序列。我们得出的结论是，菌株C29代表了一个新的Protomyces物种，并提出了P. arabidopsidicola sp。的名称。十一月 因此，我们建议原生质体不仅仅与先前公认的寄主植物严格相关。在拟南芥的叶球上可以持续存在，并且在不同国家的多个地点的拟南芥上发现了Protomyces序列。我们得出的结论是，菌株C29代表了一个新的Protomyces物种，并提出了P. arabidopsidicola sp。的名称。十一月 因此，我们建议原生质体不仅仅与先前公认的寄主植物严格相关。