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Elongase of very long chain fatty acids 6 (ELOVL6) promotes lipid synthesis in buffalo mammary epithelial cells
Journal of Animal Physiology and Animal Nutrition ( IF 2.7 ) Pub Date : 2021-03-19 , DOI: 10.1111/jpn.13536
Xinyang Fan 1 , Wei Zhu 1, 2 , Lihua Qiu 1 , Guangle Zhang 1 , Yongyun Zhang 1, 3 , Yongwang Miao 1
Affiliation  

Recent studies have shown elongase of very-long-chain fatty acids 6 (ELOVL6) is a vital protein for endogenous synthesis of saturated and monounsaturated long-chain fatty acids in some mammals. Nevertheless, its role in lipid synthesis in buffalo mammary gland is still unclear. In this work, the full-length coding sequence (CDS) of ELOVL6 was cloned and identified from buffalo mammary gland. As a result, the CDS of this gene is 795 bp, which encodes a polypeptide of 264 amino acid residues. The buffalo ELOVL6 contains an ELO domain which belongs to the ELO superfamily. Among the 10 tissues of buffalo in peak lactation detected by RT-qPCR, the expression level of ELOVL6 was the highest in the brain, followed by the spleen, and then decreased in the mammary gland, muscle, kidney, heart, liver, rumen, intestine and lung. However, only the expression in the brain and spleen was statistically different from that in other tissues (p < 0.05). Compared with that of the dry-off period, the mRNA abundance of ELOVL6 in the mammary gland was significantly increased in peak lactation. The experiments based on lentivirus transfection in buffalo mammary epithelial cells (BuMECs) displayed that the overexpression of ELOVL6 markedly promoted the expression of INSIG1, INSIG2, SREBP, PPARG, FASN, GPAM, DGAT2 and APGAT6 genes, and the knockdown of ELOVL6 significantly decreased the mRNA abundance of INSIG2, SREBP, FASN, SCD, GPAM, APGAT6 and TIP47 genes. In addition, the increase or decrease of ELOVL6 expression level also caused the corresponding change of total triglyceride content in the BuMECs. The results here suggest that the ELOVL6 can catalyse the synthesis of long-chain fatty acids in the BuMECs, and it can indirectly affect the expression of genes related to milk fat synthesis through its catalytic products to promote the lipid biosynthesis of BuMECs.

中文翻译:

超长链脂肪酸 6 (ELOVL6) 延长酶促进水牛乳腺上皮细胞中的脂质合成

最近的研究表明,超长链脂肪酸延长酶 6 (ELOVL6) 是一些哺乳动物内源性合成饱和和单不饱和长链脂肪酸的重要蛋白质。然而,其在水牛乳腺脂质合成中的作用仍不清楚。在这项工作中, ELOVL6的全长编码序列 (CDS)从水牛乳腺中克隆和鉴定。结果,该基因的CDS为795 bp,编码264个氨基酸残基的多肽。水牛 ELOVL6 包含一个属于 ELO 超家族的 ELO 结构域。RT-qPCR检测到的10个泌乳高峰水牛组织中,ELOVL6在脑中的表达水平最高,其次是脾,然后在乳腺、肌肉、肾脏、心脏、肝脏、瘤胃、肠和肺。然而,只有大脑和脾脏中的表达与其他组织中的表达有统计学差异(p  <0.05)。与枯水期相比,ELOVL6的 mRNA 丰度在哺乳高峰期乳腺中显着增加。基于慢病毒转染水牛乳腺上皮细胞 (BuMECs) 的实验表明,ELOVL6 的过表达着促进了INSIG1INSIG2SREBPPPARGFASNGPAMDGAT2APGAT6基因的表达,并且 ELOVL6 的敲低着降低了INSIG2 , SREBP , FASN , SCD , GPAM , APGAT6_TIP47基因。此外,ELOVL6表达水平的升高或降低也会引起BuMECs中总甘油三酯含量的相应变化。这里的结果表明ELOVL6可以催化BuMECs中长链脂肪酸的合成,并通过其催化产物间接影响乳脂合成相关基因的表达,从而促进BuMECs的脂质生物合成。
更新日期:2021-03-19
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