The rapid mixing of reagents is critical to a wide range of chemical and biological reactions but is difficult to implement in microfluidic devices, particularly in capillary action/passive pumping devices or in point-of-need environments. Here, we develop a self-pumping asymmetric staggered herringbone mixer made from only laser-ablated glass and tape. This lab-on-a-chip platform is capable of rapid flow (0.14 mL min⁻¹, 1 cm s⁻¹) and fast mixing (< 10 s) without external forces or pumps and is amenable to the flow of non-aqueous solvents. Furthermore, the degree of mixing and flow rates are easily tunable through the length and depth of the herringbone grooves, and the thickness of the double-sided tape that defines the channel height, respectively. The device utility is demonstrated for chemical and biological assays through the reaction of Ni(II) and DMG in ethanol/water and the enzymatic reaction of o-dianisidine with peroxidase, respectively.

试剂的快速混合对于广泛的化学和生物反应至关重要，但是很难在微流体设备中实施，尤其是在毛细管作用/被动泵送设备或需要的环境中。在这里，我们开发了一种仅由激光烧蚀的玻璃和胶带制成的自抽式不对称交错人字形混合器。该芯片实验室平台能够快速流动（0.14 mL min ^-1，1 cm s ^-1）和快速混合（<10 s），无需外力或泵，并且适合非水溶剂的流动。此外，可以通过人字形凹槽的长度和深度以及分别定义通道高度的双面胶带的厚度轻松调整混合程度和流速。分别通过Ni（II）和DMG在乙醇/水中的反应以及邻二苯胺啶与过氧化物酶的酶促反应，证明了该设备可用于化学和生物学测定。