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Comparison of 2 glucose analytical methodologies in immature Kemp’s ridley sea turtles: dry chemistry of plasma versus point-of-care glucometer analysis of whole blood
The Journal of Veterinary Diagnostic Investigation ( IF 1.5 ) Pub Date : 2021-03-19 , DOI: 10.1177/10406387211001830
Justin R Perrault 1 , Michael D Arendt 2 , Jeffrey A Schwenter 2 , Julia L Byrd 3 , Kathryn A Tuxbury 4 , Nicole I Stacy 5
Affiliation  

Blood glucose measurements provide important diagnostic information regarding stress, disease, and nutritional status. Glucose analytical methodologies include dry chemistry analysis (DCA) of plasma and point-of-care (POC) glucometer analysis of whole blood; however, these 2 methods differ in cost, required sample volume, and processing time. Because POC glucometers use built-in equations based on features of mammalian blood to convert whole blood measurements to plasma equivalent units, obtained glucose data must be compared and validated using gold-standard chemistry analytical methodology in reptiles. For in-water, trawl-captured, immature Kemp’s ridley sea turtles (Lepidochelys kempii) from Georgia, USA, we observed significant, positive agreement between the 2 glucose determination methods; however, the glucometer overestimated glucose concentrations by 1.4 mmol/L on average in comparison to DCA and produced a wider range of results. The discordance of these results suggests that POC glucometer glucose data should be interpreted in the context of methodology- and brand-specific reference intervals along with concurrent packed cell volume data.



中文翻译:

未成熟的肯普氏雷德利海龟中两种葡萄糖分析方法的比较:血浆的干化学与全血的即时血糖仪分析

血糖测量提供有关压力、疾病和营养状况的重要诊断信息。葡萄糖分析方法包括血浆的干化学分析 (DCA) 和全血的即时 (POC) 血糖仪分析;但是,这两种方法在成本、所需样本量和处理时间方面有所不同。由于 POC 血糖仪使用基于哺乳动物血液特征的内置方程将全血测量值转换为血浆等效单位,因此必须使用爬行动物的黄金标准化学分析方法对获得的葡萄糖数据进行比较和验证。适用于水中拖网捕获的未成熟肯普氏雷德利海龟 ( Lepidochelys kempii) 来自美国乔治亚州,我们观察到两种葡萄糖测定方法之间存在显着的阳性一致性;然而,与 DCA 相比,血糖仪平均高估了 1.4 mmol/L 的葡萄糖浓度,并产生了更广泛的结果。这些结果的不一致表明,POC 血糖仪血糖数据应在方法学和品牌特定的参考区间以及同时打包的细胞体积数据的背景下进行解释。

更新日期:2021-03-19
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