This study was conducted to isolate and identify extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacterales in conventional and organic chicken meats, which were sold in Turkey. A total of 200 raw chicken meat sample (100 conventional and 100 organic) were used as material. Classic culture technique based on chromogenic method was used for the isolation of bacteria, and the identification was performed with VITEK MS. Phenotypic ESBL production was detected by combined disc diffusion method. Gene regions responsible for ESBL production were determined by PCR. MIC values of isolates were detected by VITEK 2. Phenotypic ESBL‐producing Enterobacterales were detected in 46% of conventional chicken meats and in 22% of organic chicken meats. Of the 115 isolates obtained, 97 (84%) were Escherichia coli, 12 (10%) were Klebsiella pneumoniae, four (3·48%) were Serratia fonticola, one (0·87%) was Rahnella aquatilis, and one (0·87%) was Serratia liquefaciens. PCR analysis revealed that 109 of 115 isolates (94·78%) contained at least one of the bla_CTX‐M, bla_TEM, and bla_SHV genes. Of the 115 ESBL‐producing isolates, 103 (89·57%) were found resistant to at least one antibiotic except for the β‐lactam group. The contamination level of ESBL‐producing Enterobacterales was higher in conventional chicken meats (P < 0·001).

中文翻译：

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用有机和常规鸡肉生产广谱β-内酰胺酶肠杆菌的特性

进行这项研究的目的是分离和鉴定在土耳其出售的常规和有机鸡肉中产生超广谱β-内酰胺酶（ESBL）的肠杆菌。总共使用200个生鸡肉样品（100个常规鸡肉样品和100个有机鸡肉样品）作为材料。使用基于生色法的经典培养技术分离细菌，并使用VITEK MS进行鉴定。通过组合圆盘扩散法检测表型ESBL的产生。通过PCR确定负责ESBL产生的基因区域。通过VITEK 2检测到了分离物的MIC值。在46％的常规鸡肉和22％的有机鸡肉中检测到产生表型ESBL的肠杆菌。在获得的115株分离物中，有97株（84％）是大肠杆菌其中，肺炎克雷伯菌有12（10％）种，是沙雷氏菌（Serratia fonticola）有4（3·48％），水生拉氏菌（Rahnella aquatilis）有1（0·87％），而液化沙雷氏菌有1（0·87％）。PCR分析表明，在115株分离株中，有109株（94·78％）含有bla _CTX-M，bla _TEM和bla _SHV基因中的至少一种。在115株产生ESBL的分离物中，除β-内酰胺组外，发现有103株（89·57％）对至少一种抗生素具有抗药性。传统鸡肉中产生ESBL的肠杆菌的污染水平较高（P  <0·001）。