Irradiation of tumor cell lines is a useful way to investigate the effects of ionizing radiation on biological molecules. We designed an easy and reproducible approach for in vitro experimental high dose rate brachytherapy, which was simulated by a Monte Carlo code and dosimetrically characterized by experimental methods to evaluate the correspondence between planned doses and doses absorbed by the cells. This approach is an acrylic platform containing T25 tissue culture flasks and multiwell tissue culture plates. It allows nine parallel needles carrying an ¹⁹²Ir source to irradiate the adherent cells. The whole system composed of the acrylic platform, tissue culture flasks and ¹⁹²Ir source tracking was simulated by the Monte Carlo N-Particle transport code (MCNPX). Dosimetric measurements were taken by well ionization chamber and radiochromic films. There was a slight difference, averaging from 2% to 7%, between the MCNPX results and film dosimetry results regarding uniform radiation created by the source arrangement. The results showed different values for planned and measured doses in each cell culture plate, which was attributed to the non-equivalent water material used and to the lack of full scattering coming from the top of the platform. This last contribution was different for each tissue culture plate and an individual dose correction factor was calculated. The dose correction factor must be applied to match the planned dose and the actual doses absorbed by the cells. The designed approach is an efficient tool for in vitro brachytherapy experiments for most commercial cell culture plates.

肿瘤细胞系的辐射是研究电离辐射对生物分子的影响的有用方法。我们为体外实验性高剂量率近距离放射治疗设计了一种简单且可重复的方法，该方法由蒙特卡罗代码模拟，并通过实验方法进行剂量学表征，以评估计划剂量与细胞吸收剂量之间的对应关系。这种方法是一个包含 T25 组织培养瓶和多孔组织培养板的丙烯酸平台。它允许携带¹⁹² Ir 源的九个平行针照射贴壁细胞。整个系统由亚克力平台、组织培养瓶和¹⁹²红外源跟踪由蒙特卡罗 N 粒子传输代码 (MCNPX) 模拟。通过井电离室和辐射变色膜进行剂量测定。在 MCNPX 结果和胶片剂量测定结果之间存在轻微差异，平均为 2% 到 7%，关于源排列产生的均匀辐射。结果显示每个细胞培养板中计划和测量的剂量值不同，这归因于使用的非等效水材料以及缺乏来自平台顶部的完全散射。每个组织培养板的最后贡献是不同的，并且计算了单独的剂量校正因子。必须应用剂量校正因子以匹配计划剂量和细胞吸收的实际剂量。