The nitrification process in recirculating aquaculture systems can reduce water pH. Fish can also be exposed to water acidification during transport, an important feature in the aquarium industry, as live fish can be kept in a closed environment for more than 24 h during overseas aerial transportation. Therefore, it is important to study the responses of fish to acidic environments. We investigated the impacts of acute exposure to decreasing pH levels in orange clownfish Amphiprion percula juveniles on their survival and oxidative stress status. Fish were exposed to pH 5, 6, 7, and 8 for 96 h. We observed a significant reduction in survival (85%) and protein damage as measured by P-SH (protein thiol) for fish maintained at pH 5. Despite no effects on survival or oxidative damage, fish exposed to pH 6 showed an increase in their antioxidant defense systems, demonstrating this pH level could not be suitable for them as well. Furthermore, there were no negative effects for fish kept at pH 7, compared to those maintained at pH 8 during this short-term evaluation.

循环水产养殖系统中的硝化过程会降低水的 pH 值。鱼在运输过程中也可能暴露于水酸化，这是水族馆行业的一个重要特征，因为在海外航空运输过程中，活鱼可以在密闭环境中保存 24 小时以上。因此，研究鱼类对酸性环境的反应非常重要。我们研究了急性暴露对橙色小丑鱼Amphiprion percula降低 pH 值的影响幼鱼的生存和氧化应激状态。将鱼暴露于 pH 值 5、6、7 和 8 中 96 小时。我们观察到维持在 pH 5 的鱼的存活率 (85%) 和蛋白质损伤显着降低 (85%)。抗氧化防御系统，表明这种 pH 值水平也不适合他们。此外，与在短期评估期间保持在 pH 值 8 的鱼相比，保持在 pH 值 7 的鱼没有负面影响。