In this study we investigated the secA gene as possible marker to supplement the classification scheme based on 16S rRNA sequences. We cloned and sequenced secA gene and its flanking region of ‘Ca. P. asteris’ CPh strain phytoplasma, as well as 1997 bp length secA gene fragment of Canada peach X-disease CX and 1327 bp fragment of ‘Ca. P. ziziphi’ JWB. This helped us to select a primer pair for a single step PCR that can amplify ~1200 kb length fragment of secA gene from different phytoplasma groups. We propose that this fragment could be used in RFLP analysis to quickly identify and distinguish 16SrI-A, I-B, I-C, I-D, III-A, III-B, III-E, III-F, III-H, V-B, XII-B and XXI-A subgroup phytoplasmas using just two restriction endonucleases.

在这项研究中，我们调查了secA基因作为可能的标记，以补充基于16S rRNA序列的分类方案。我们克隆和测序SECA基因和'它的侧翼区钙。P.阿斯特里斯“器CPh应变植原体，以及1997个碱基长度SECA加拿大桃X-CX疾病的基因片段和的1327 bp片段”钙。P. ziziphi'JWB。这有助于我们为一步PCR选择引物对，该引物对可以扩增约1200 kb长的secA片段来自不同植物质群的基因。我们建议将该片段用于RFLP分析，以快速识别和区分16SrI-A，IB，IC，ID，III-A，III-B，III-E，III-F，III-H，VB，XII-仅使用两个限制性核酸内切酶的B和XXI-A亚类植物质浆。