Guidance errors and unrefined neural map configurations appear linked to certain neurodevelopmental conditions, including autism spectrum disorders. Deficits in specific multisensory tasks that require midbrain processing are highly predictive of cognitive and behavioral phenotypes associated with such syndromes. The lateral cortex of the inferior colliculus (LCIC) is a shell region of the mesencephalon that integrates converging information from multiple levels and modalities. Mature LCIC sensory maps are discretely-organized, mimicking its compartmental micro-organization. Intermittent modular domains receive patchy somatosensory connections, while inputs of auditory origin terminate in the encompassing extramodular matrix. Eph-ephrin signaling mechanisms instruct comparable topographic arrangements in a variety of other systems. Whether Eph-ephrin interactions also govern the assembly of LCIC multimodal maps remains unaddressed. Previously, we identified EphA4 and ephrin-B2 as key mediators, with overlapping expression patterns that align with emerging LCIC modules. Here, we implicate another member of this guidance family, ephrin-B3, and quantify its transient expression with respect to neurochemically-defined LCIC compartments. Multiple-labeling studies in GAD67-GFP knock-in mice reveal extramodular ephrin-B3 expression, complementary to that of EphA4 and ephrin-B2. This distinctive pattern sharpens over the early postnatal period (birth to P8), prior to ephrin-B3 downregulation once multimodal LCIC inputs are largely segregated (P12). Channel-specific sampling of LCIC ROIs show ephrin-B3 signal periodicities that are out-of-phase with GAD (modular marker) signal fluctuations, and match calretinin (CR) waveforms (matrix marker). Taken together, the guidance mosaic registry with emerging LCIC compartments and its interfacing afferent streams suggest a prominent role for Eph-ephrins in ordering behaviorally significant multisensory midbrain networks.

中文翻译：

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关于新兴的多峰中脑图的室Ephrin-B3指导模式的注册表。

指导错误和未完善的神经图配置似乎与某些神经发育状况（包括自闭症谱系障碍）有关。需要中脑处理的特定的多感官任务的缺陷可以高度预测与此类综合征相关的认知和行为表型。下丘脑外侧皮质（LCIC）是中脑的壳区域，其整合来自多个级别和方式的会聚信息。成熟的LCIC感官图是离散组织的，模仿了其隔室的微观组织。间歇性模块化域接收斑驳的体感连接，而听觉起源的输入终止于包围的模块化外矩阵中。eph-ephrin信号传导机制指示各种其他系统中可比的地形布置。Eph-ephrin相互作用是否也控制LCIC多峰图的组装尚不清楚。以前，我们将EphA4和ephrin-B2确定为关键介体，具有与新兴LCIC模块对齐的重叠表达模式。在这里，我们暗示了该指导家族的另一个成员，ephrin-B3，并量化了其相对于神经化学定义的LCIC区室的瞬时表达。在GAD67-GFP敲入小鼠中的多标记研究表明，与EphA4和ephrin-B2互补的ephrin-B3表达在模块外。一旦多式联运的LCIC投入大量隔离（P12），这种独特的模式会在出生后早期（出生至P8），在ephrin-B3下调之前加剧。LCIC ROI的通道特定采样显示，ephrin-B3信号周期与GAD（模块化标记）信号波动异相，并且与钙网蛋白（CR）波形（矩阵标记）匹配。两者合计，新兴的LCIC隔室及其接口传入的流的引导镶嵌注册表表明，麻黄素在订购行为上重要的多感觉中脑网络方面起着重要作用。