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The neurotransmitter receptor architecture of the mouse olfactory system
Frontiers in Neuroanatomy ( IF 2.9 ) Pub Date : 2021-03-03 , DOI: 10.3389/fnana.2021.632549
Kimberley Lothmann , Katrin Amunts , Christina Herold

The uptake, transmission and processing of sensory olfactory information is modulated by inhibitory and excitatory receptors in the olfactory system. Previous studies have focused on the function of individual receptors in distinct brain areas, but the receptor architecture of the whole system remains unclear. Here, we analyzed the receptor profiles of the whole olfactory system of adult male mice. We examined the distribution patterns of glutamatergic (AMPA, kainate, mGlu2/3 and NMDA), GABAergic (GABAA, GABAA(BZ) and GABAB), dopaminergic (D1/5) and noradrenergic (α1 and α2) neurotransmitter receptors by quantitative in vitro receptor autoradiography combined with an analysis of the cyto- and myelo-architecture. We observed that each subarea of the olfactory system is characterized by individual densities of distinct neurotransmitter receptor types, leading to a region- and layer-specific receptor profile. Thereby, the investigated receptors in the respective areas and strata showed a heterogeneous expression. Generally, we detected high densities of mGlu2/3Rs, GABAA(BZ)Rs and GABABRs. Noradrenergic receptors revealed a highly heterogenic distribution, while the dopaminergic receptor D1/5 displayed low concentrations, except in the olfactory tubercle and the dorsal endopiriform nucleus. The similarities and dissimilarities of the area-specific multireceptor profiles were analyzed by a hierarchical cluster analysis. A three-cluster solution was found that divided the areas into the (1) olfactory relay stations (main and accessory olfactory bulb), (2) the olfactory cortex (anterior olfactory cortex, dorsal peduncular cortex, taenia tecta, piriform cortex, endopiriform nucleus, entorhinal cortex, orbitofrontal cortex) and the (3) olfactory tubercle, constituting its own cluster. The multimodal receptor-architectonic analysis of each component of the olfactory system provides new insights into its neurochemical organization and future possibilities for pharmaceutic targeting.

中文翻译:

小鼠嗅觉系统的神经递质受体结构

嗅觉信息的吸收,传递和处理受嗅觉系统中的抑制性和兴奋性受体调节。先前的研究集中于各个受体在不同大脑区域的功能,但整个系统的受体结构仍不清楚。在这里,我们分析了成年雄性小鼠的整个嗅觉系统的受体概况。我们通过定量体外检测了谷氨酸能(AMPA,海藻酸酯,mGlu2 / 3和NMDA),GABA能(GABAA,GABAA(BZ)和GABAB),多巴胺能(D1 / 5)和去甲肾上腺素能(α1和α2)神经递质受体的分布模式受体放射自显影结合细胞和骨髓结构的分析。我们观察到,嗅觉系统的每个子区域的特征在于不同的神经递质受体类型的个体密度,从而导致区域和层特异性受体分布。因此,在各个区域和地层中研究的受体显示出异质表达。通常,我们检测到高密度的mGlu2 / 3Rs,GABAA(BZ)Rs和GABABRs。去甲肾上腺素能受体显示出高度异质的分布,而多巴胺能受体D1 / 5则显示低浓度,除了在嗅结节和背侧内膜状核中。通过分层聚类分析,分析了区域特异性多受体谱的相似性和相异性。发现了一种三类解决方案,将区域分为(1)个嗅觉中继站(主嗅球和辅助嗅球),(2)嗅觉皮层(嗅觉前皮层,背侧椎弓根皮层,Taenia tecta,梨状皮层,内膜状核,内嗅皮层,眶额叶皮层)和(3)嗅结节,构成​​其自身的簇。嗅觉系统各组成部分的多峰受体-建筑分析为神经化学组织和药物靶向的未来可能性提供了新的见解。
更新日期:2021-03-17
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