Background. MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear. Methods. In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test. Results. The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS. Conclusion. The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC.

中文翻译：

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MicroRNA-497-5p 在肝细胞癌中下调并与患者的肿瘤发生和预后不良有关

背景。MicroRNAs (miRNAs) 已被证明在包括肝细胞癌 (HCC) 在内的多种恶性肿瘤中表现出重要的调节作用。据报道，hsa-miR-497-5p 与多种肿瘤的癌症进展和预后不良有关。然而，hsa-miR-497-5p在HCC中的表达及其临床意义仍不清楚。方法. 在本研究中，我们研究了 hsa-miR-497-5p 在 HCC 中的表达，并分析了临床特征与预后的校正。使用癌症基因组图谱 (TCGA) 数据库和基因表达综合 (GEO) 数据集在 HCC 和邻近非癌组织中分析 hsa-miR-497-5p 和潜在靶基因的表达水平。实时定量逆转录聚合酶链反应 (qRT-PCR) 用于分析 328 例 HCC 组织和 30 对相邻非癌组织中的 hsa-miR-497-5p 水平。使用 Kaplan-Meier 方法和时序检验评估 HCC 患者的总生存期 (OS) 和无进展生存期 (PFS)。结果. 与正常组织相比，HCC组织中hsa-miR-497-5p表达水平降低，其靶基因ACTG1、CSNK1D、PPP1CC和BIRC5上调。较低水平的 hsa-miR-497-5p 表达和较高水平的四种靶基因与较高的肿瘤直径显着相关。此外，具有较低 hsa-miR-497-5p 表达和较高靶基因水平的患者 OS 较短。结论。hsa-miR-497-5p的表达水平可能在HCC中发挥重要的调节作用，与HCC的进展和患者的不良预后密切相关。hsa-miR-497-5p 可能是治疗 HCC 的特异性治疗靶点。