Lotus spp. are a plant of great importance as a forage to improve pastures, to restore degraded soils and the inoculation with best rhizobial strains is widely used in many countries. The aim of this work was to analyze the phylogeny of bacteria nodulating Lotus creticus collected from sandy alkaline saline soil in Tangier province Northern Morocco and determine their identity. Out of 44 bacterial isolates, 21 strains were selected for the phenotypic characterization and symbiotic efficiency evaluation. The enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR) fingerprinting showed different patterns at 40% similarity, and the amplified ribosomal DNA restriction analysis (ARDRA) generated one ribotype. Based on both the ERIC-PCR and the ARDRA results, 2 isolates, LcT51 and LcA30, were then chosen for molecular analysis. The 16S rRNA gene sequences showed that the 2 strains are closely related to Ensifer meliloti LMG 6133^T with similarity values of 99.69%% to 99.93%. The analysis of concatenated atpD, glnII, gyrB, rpoB and dnaK housekeeping genes sequences confirmed the affiliation of the two strains to E. meliloti LMG 6133^T. The phylogenic analysis of the nodC and nifH symbiotic genes, confirmed that L. creticus is nodulated in Tangier alkaline soils by the symbiovar lancerottense of E. meliloti.

莲花属 作为改良牧场的草料，恢复退化土壤的植物非常重要，接种最佳根瘤菌的疫苗已在许多国家广泛使用。这项工作的目的是分析结节莲花的细菌的系统发育从摩洛哥北部丹吉尔省的含沙碱性盐土中收集并确定其身份。从44个细菌分离物中，选择21个菌株用于表型表征和共生效率评估。肠细菌重复基因间共有（ERIC）-聚合酶链反应（PCR）指纹图谱显示出40％相似的不同模式，而扩增的核糖体DNA限制性内切酶分析（ARDRA）产生了一种核糖型。根据ERIC-PCR和ARDRA结果，然后选择2个分离株LcT51和LcA30进行分子分析。16S rRNA基因序列表明，这2个菌株均与Ensifer meliloti LMG 6133 ^T密切相关，相似度在99.69 %%至99.93％之间。级联atpD的分析，glnII，gyrB，rpoB和dnaK管家基因序列证实了这两个菌株与大肠杆菌E. meliloti LMG 6133 ^T的隶属关系。所述的系统发育分析NODC和nifH共生基因，确认L. creticus通过的symbiovar lancerottense丹吉尔碱性土壤根瘤E.根瘤菌。