Background Neuroinflammation is important in the pathophysiology of spontaneous intracerebral hemorrhage (ICH) and peripheral inflammatory cells play a role in the clinical evolution and outcome. Methodology Blood samples from ICH patients ( n = 20) were collected at admission for 5 consecutive days for peripheral blood mononuclear cells (PBMCs). Frozen PBMCs were used for real-time PCR using Taqman probes (NFKB1, SOD1, PPARG, IL10, NFE2L2, and REL) and normalized to GAPDH. Data on hospital length of stay and modified Rankin score (MRS) were collected with 90-day MRS ≤ 3 as favorable outcome. Statistical analysis of clinical characteristics to temporal gene expression from early to delayed timepoints was compared for MRS groups (favorable vs unfavorable) and hematoma volume. Principle findings and results IL10, SOD1, and REL expression were significantly higher at delayed timepoints in PBMCs of ICH patients with favorable outcome. PPARG and REL increased between timepoints in patients with favorable outcome. NFKB1 expression was not sustained, but significantly decreased from higher levels at early onset in patients with unfavorable outcome. IL10 expression showed a negative correlation in patients with high hematoma volume (>30 mL). Conclusions and significance Anti-inflammatory, pro-survival regulators were highly expressed at delayed time points in ICH patients with a favorable outcome, and IL10 expression showed a negative correlation to high hematoma volume.

中文翻译：

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自发性脑出血后PBMC中的抗炎基因

背景神经炎症在自发性脑出血 (ICH) 的病理生理学中很重要，外周炎症细胞在临床演变和结果中发挥作用。方法学 ICH 患者 (n = 20) 的血液样本在入院时连续 5 天收集用于外周血单个核细胞 (PBMC)。使用 Taqman 探针（NFKB1、SOD1、PPARG、IL10、NFE2L2 和 REL）将冷冻 PBMC 用于实时 PCR，并标准化为 GAPDH。以 90 天 MRS ≤ 3 为有利结果收集了住院时间和改良 Rankin 评分 (MRS) 的数据。比较了 MRS 组（有利与不利）和血肿体积的临床特征对从早期到延迟时间点的时间基因表达的统计分析。主要发现和结果 IL10、SOD1、ICH 患者 PBMC 的延迟时间点和 REL 表达显着更高，结果良好。PPARG 和 REL 在具有良好结果的患者的时间点之间增加。NFKB1 表达不可持续，但在预后不良的患者中较早发时的较高水平显着下降。IL10 表达在血肿体积高（>30 mL）的患者中呈负相关。结论和意义 抗炎、促生存调节因子在 ICH 患者延迟时间点高度表达，结果良好，IL10 表达与高血肿体积呈负相关。NFKB1 表达不可持续，但在预后不良的患者中较早发时的较高水平显着下降。IL10 表达在血肿体积高（>30 mL）的患者中呈负相关。结论和意义 抗炎、促生存调节因子在 ICH 患者延迟时间点高度表达，结果良好，IL10 表达与高血肿体积呈负相关。NFKB1 表达不可持续，但在预后不良的患者中较早发时的较高水平显着下降。IL10 表达在血肿体积高（>30 mL）的患者中呈负相关。结论和意义 抗炎、促生存调节因子在 ICH 患者延迟时间点高度表达，结果良好，IL10 表达与高血肿体积呈负相关。