Different phytochemicals from various plant species exhibit promising medicinal properties against cancer. Juniperus phoenicea is a plant species that has been found to present medicinal properties. Herein, crude extract and fractions of J. phoenicea were examined to determine its anticancer properties against several cancer cells. The active fraction was chosen to assess its activity on cell cycle progression and apoptosis induction by annexin and propidium iodide (PI) biomarkers. Further, phytochemical screening for possible contents of active fraction using gas chromatography–mass spectrometry (GC-MS) analysis was conducted. It was demonstrated that cell proliferation was suppressed, and the MCF-7 cell line was the most sensitive to J. phoenicea chloroform fraction (JPCF), with the IC 50 values of 24.5 μg/mL. The anti-proliferation activity of JPCF in MCF-7 cells was linked to the aggregation of cells in the G1 phase, increases in early and late apoptosis as well as necrotic cell death. Contents analysis of JPCF using GC-MS analysis identified 3-methyl-5-(2′,6′,6′-trimethylcyclohex-1′-enyl)-1-penten-3-ol (16.5%), methyl 8-oxooctanoate (15.61%), cubenol (13.48%), and 7-oxabicyclo [2.2.1] heptane (12.14%) as major constituents. Our present study provides clear evidence that J. phoenicea can inhibit cell proliferation, trigger cell cycle arrest, and induce apoptosis in tested cancer cells.

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刺柏叶绿素诱导细胞凋亡和细胞周期阻滞及化学成分分析

来自各种植物物种的不同植物化学物质显示出有希望的抗癌医学特性。凤头杜鹃是一种已发现具有药用特性的植物。在本文中，检查了粗制的提取物和凤梨的部分，以确定其对几种癌细胞的抗癌特性。选择活性级分以通过膜联蛋白和碘化丙啶（PI）生物标记物评估其对细胞周期进程和细胞凋亡诱导的活性。此外，还进行了使用气相色谱-质谱（GC-MS）分析进行植物化学筛选，以分析活性成分的可能含量。结果表明，细胞增殖受到抑制，MCF-7细胞系对腓肠杆菌氯仿组分（JPCF）最敏感，IC 50值为24.5μg/ mL。JPCF在MCF-7细胞中的抗增殖活性与G1期细胞的聚集，早期和晚期凋亡以及坏死细胞死亡的增加有关。使用GC-MS分析JPCF的含量，鉴定出3-甲基-5-（2'，6'，6'-三甲基环己基-1'-烯基）-1-戊烯-3-醇（16.5％），8-氧代辛酸甲酯（15.61％），立方酚（13.48％）和7-氧杂双环[2.2.1]庚烷（12.14％）为主要成分。我们目前的研究提供了明确的证据，表明凤凰氏假单胞菌可以抑制细胞增殖，触发细胞周期停滞并诱导被测癌细胞的凋亡。主要成分为8％的氧代辛酸甲酯（15.61％），立方酚（13.48％）和7-氧杂双环[2.2.1]庚烷（12.14％）。我们目前的研究提供了明确的证据，表明凤凰氏假单胞菌可以抑制细胞增殖，触发细胞周期停滞并诱导被测癌细胞的凋亡。主要成分为8％的氧代辛酸甲酯（15.61％），立方酚（13.48％）和7-氧杂双环[2.2.1]庚烷（12.14％）。我们目前的研究提供了明确的证据，表明凤凰氏假单胞菌可以抑制细胞增殖，触发细胞周期停滞并诱导被测癌细胞的凋亡。