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Differential effects of intense exercise and pollution on the airways in a murine model
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2021-03-15 , DOI: 10.1186/s12989-021-00401-6 Tatjana Decaesteker 1 , Eliane Vanhoffelen 2 , Kristel Trekels 2 , Anne-Charlotte Jonckheere 3 , Jonathan Cremer 3 , Arno Vanstapel 1 , Ellen Dilissen 3 , Dominique Bullens 3, 4 , Lieven J Dupont 1, 5 , Jeroen A Vanoirbeek 2
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2021-03-15 , DOI: 10.1186/s12989-021-00401-6 Tatjana Decaesteker 1 , Eliane Vanhoffelen 2 , Kristel Trekels 2 , Anne-Charlotte Jonckheere 3 , Jonathan Cremer 3 , Arno Vanstapel 1 , Ellen Dilissen 3 , Dominique Bullens 3, 4 , Lieven J Dupont 1, 5 , Jeroen A Vanoirbeek 2
Affiliation
Exercise-induced bronchoconstriction (EIB) is a transient airway narrowing, occurring during or shortly after intensive exercise. It is highly prevalent in non-asthmatic outdoor endurance athletes suggesting an important contribution of air pollution in the development of EIB. Therefore, more research is necessary to investigate the combination of exercise and pollutants on the airways. Balbc/ByJ mice were intranasally challenged 5 days a week for 3 weeks with saline or 0.2 mg/ml diesel exhaust particles (DEP), prior to a daily incremental running session or non-exercise session. Once a week, the early ventilatory response was measured and lung function was determined at day 24. Airway inflammation and cytokine levels were evaluated in bronchoalveolar lavage fluid. Furthermore, innate lymphoid cells, dendritic cells and tight junction mRNA expression were determined in lung tissue. Submaximal exercise resulted in acute alterations of the breathing pattern and significantly improved FEV0.1 at day 24. DEP exposure induced neutrophilic airway inflammation, accompanied with increased percentages of CD11b+ DC in lung tissue and pro-inflammatory cytokines, such as IL-13, MCP-1, GM-CSF and KC. Occludin and claudin-1(Cldn-1) expression were respectively increased and decreased by DEP exposure. Whereas, exercise increased Cldn-3 and Cldn-18 expression. Combining exercise and DEP exposure resulted in significantly increased SP-D levels in the airways. DEP exposure induced typical airway neutrophilia, DC recruitment and pro-inflammatory cytokine production. Whereas, intensive exercise induced changes of the breathing pattern. The combination of both triggers resulted in a dysregulation of tight junction expression, suggesting that intensive exercise in polluted environments can induce important changes in the airway physiology and integrity.
中文翻译:
剧烈运动和污染对小鼠模型气道的不同影响
运动诱发的支气管收缩 (EIB) 是一种短暂的气道狭窄,发生在剧烈运动期间或之后不久。它在非哮喘户外耐力运动员中非常普遍,这表明空气污染在 EIB 的发展中起着重要作用。因此,需要更多的研究来调查运动和污染物对呼吸道的影响。Balbc/ByJ 小鼠每周 5 天,连续 3 周用盐水或 0.2 毫克/毫升柴油机尾气颗粒 (DEP) 进行鼻内攻击,然后每天增量跑步或非锻炼。每周一次,测量早期通气反应并在第 24 天测定肺功能。在支气管肺泡灌洗液中评估气道炎症和细胞因子水平。此外,先天淋巴细胞,在肺组织中测定了树突细胞和紧密连接 mRNA 的表达。次极量运动导致呼吸模式的急性改变,并在第 24 天显着改善 FEV0.1。 DEP 暴露诱导中性粒细胞气道炎症,伴随着肺组织中 CD11b+ DC 和促炎细胞因子(如 IL-13、MCP)的百分比增加-1,GM-CSF 和 KC。Occludin 和claudin-1(Cldn-1) 表达分别增加和减少DEP 暴露。而运动会增加 Cldn-3 和 Cldn-18 的表达。结合运动和 DEP 暴露导致气道中 SP-D 水平显着增加。DEP 暴露诱导典型的气道中性粒细胞增多、DC 募集和促炎细胞因子的产生。然而,剧烈运动会引起呼吸模式的变化。
更新日期:2021-03-15
中文翻译:
剧烈运动和污染对小鼠模型气道的不同影响
运动诱发的支气管收缩 (EIB) 是一种短暂的气道狭窄,发生在剧烈运动期间或之后不久。它在非哮喘户外耐力运动员中非常普遍,这表明空气污染在 EIB 的发展中起着重要作用。因此,需要更多的研究来调查运动和污染物对呼吸道的影响。Balbc/ByJ 小鼠每周 5 天,连续 3 周用盐水或 0.2 毫克/毫升柴油机尾气颗粒 (DEP) 进行鼻内攻击,然后每天增量跑步或非锻炼。每周一次,测量早期通气反应并在第 24 天测定肺功能。在支气管肺泡灌洗液中评估气道炎症和细胞因子水平。此外,先天淋巴细胞,在肺组织中测定了树突细胞和紧密连接 mRNA 的表达。次极量运动导致呼吸模式的急性改变,并在第 24 天显着改善 FEV0.1。 DEP 暴露诱导中性粒细胞气道炎症,伴随着肺组织中 CD11b+ DC 和促炎细胞因子(如 IL-13、MCP)的百分比增加-1,GM-CSF 和 KC。Occludin 和claudin-1(Cldn-1) 表达分别增加和减少DEP 暴露。而运动会增加 Cldn-3 和 Cldn-18 的表达。结合运动和 DEP 暴露导致气道中 SP-D 水平显着增加。DEP 暴露诱导典型的气道中性粒细胞增多、DC 募集和促炎细胞因子的产生。然而,剧烈运动会引起呼吸模式的变化。