Large-volumetric optical tomography with molecular specificity has long been pursued to tackle the challenge of dissecting both structural and chemical organization of complicated biological tissues. Here, based on counterpropagating femtosecond pulse laser trains and stimulated Raman scattering, we report pulse-sheet chemical tomography (PCT), which allows label-free bond-selective three-dimensional imaging of large intact tissues. To prove the concept, we demonstrate vibrational tomography of highly scattering bone tissue with lateral resolution of 16.4 µm and axial resolution of 24.5 µm, over a large field of view of ${8} \times {8} \times {1.6}\;{{\rm mm}^3}$ in a mouse skull with scalp. PCT resolves the trade-off between focal depth and spatial resolution, and offers unique biomedical and clinical prospects for optical tomography of tissues and organs in the future.

中文翻译：

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通过反向传播受激拉曼散射进行脉冲片层化学层析成像

长期以来，具有分子特异性的大体积光学层析成像技术一直致力于解决解剖复杂生物组织的结构和化学组织的挑战。在这里，基于反向传播的飞秒脉冲激光序列和受激拉曼散射，我们报告了脉冲片层化学层析成像（PCT），该成像片可对大型完整组织进行无标记的键选择性三维成像。为了证明这一概念，我们展示了高度散射的骨组织的层析层析成像技术，其横向分辨率为16.4 µm，轴向分辨率为24.5 µm，视野范围为$ {8} \ time {8} \ times {1.6} \; {{\ rm mm} ^ 3} $在头皮的老鼠头骨中。PCT解决了焦深和空间分辨率之间的折衷问题，并为将来的组织和器官的光学层析成像技术提供了独特的生物医学和临床前景。