There has been an increase in the incidence of hypopharyngeal and cervical esophageal cancer worldwide, and hence growing needs for hypopharyngeal and cervical esophageal tissue repair. This work produced a bi-layer composite scaffold with decellularized small intestine submucosa and polylactic-co-glycolic acid, which resembled the layered architectures of its intended tissues. The decellularized small intestine submucosa contained minimal residual DNA (52.5 ± 1.2 ng/mg) and the composite scaffold exhibited satisfactory mechanical properties (a tensile modulus of 21.1 ± 4.8 MPa, an ultimate tensile strength of 14.0 ± 2.9 MPa and a failure strain of 26.9 ± 5.1%). The interactions between cells and the respective layers of the scaffold were characterized by CCK-8 assays, immunostaining and Western blotting. Desirable cell proliferation and phenotypic behaviors were observed. These results have provided an important basis for the next-step in vivo studies of the scaffold, and bode well for its future clinical applications.

中文翻译：

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脱细胞小肠黏膜下层/聚乳酸​​-乙醇酸共聚物复合支架在下咽和颈部食管组织修复中的潜在应用

世界范围内下咽癌和颈段食管癌的发病率有所增加，因此对下咽和颈段食管组织修复的需求不断增长。这项工作产生了一种双层复合支架，具有脱细胞小肠粘膜下层和聚乳酸-共-乙醇酸，类似于其预期组织的分层结构。脱细胞的小肠黏膜下层含有最少的残留 DNA (52.5 ± 1.2 ng/mg)，复合支架表现出令人满意的机械性能（拉伸模量为 21.1 ± 4.8 MPa，极限拉伸强度为 14.0 ± 2.9 MPa，失效应变为 26.9 ± 5.1%）。细胞与支架各层之间的相互作用通过CCK-8测定、免疫染色和蛋白质印迹来表征。观察到理想的细胞增殖和表型行为。这些结果为下一步支架的体内研究提供了重要依据，并为其未来的临床应用提供了良好的预兆。