Abstract

Adhesion capacity on biological surfaces and biofilm formation is considered an important step in the infection process by Candida albicans. The ability of (PhSe)₂ and (pCl-PhSe)₂, two synthetic organic selenium (organochalcogen) compounds, to act on C. albicans virulence factors related to adhesion to human endocervical (HeLa) cell surfaces and their anti-biofilm activities was analyzed. Both organochalcogen compounds inhibited C. albicans adhesion to HeLa cells, dependent on compound concentrations. (PhSe)₂ (at 20 µM; p = 0.0012) was significantly more effective than (pCl-PhSe)₂ (at 20 µM; p = 0.0183) compared with the control. (PhSe)₂ inhibited biofilm formation and decreased biofilm viability in both early and mature biofilms more efficiently than (pCl-PhSe)₂. Overall, the organochalcogen compounds, especially (PhSe)₂, were demonstrated to be effective antifungal drugs against C. albicans virulence factors related to epithelial cell surface adhesion and the formation and viability of biofilms.

摘要

生物表面上的粘附能力和生物膜形成被认为是白色念珠菌感染过程中的重要步骤。（PhSe）₂和（p Cl-PhSe）_2这两种合成的有机硒（有机硫）化合物对白色念珠菌毒力因子的作用能力，该毒力因子与人宫颈内膜（HeLa）细胞表面的粘附及其抗生物膜活性有关被分析了。取决于化合物的浓度，两种有机硫属化合物均抑制白色念珠菌粘附于HeLa细胞。（PhSe）₂（在20 µM；p  = 0.0012）比（p Cl-PhSe）₂更有效（在20 µM；p  = 0.0183）与对照相比。（PhSe）₂比（p Cl-PhSe）₂更有效地抑制了早期和成熟生物膜中生物膜的形成并降低了生物膜的生存能力。总体而言，有机硫属化合物，尤其是（PhSe）₂被证明是有效的抗真菌药物，与白色念珠菌的致病因子有关，这些致病因子与上皮细胞表面粘附以及生物膜的形成和生存能力有关。