当前位置: X-MOL 学术Nanotoxicology › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Mutagenicity of silver nanoparticles evaluated using whole-genome sequencing in mouse lymphoma cells
Nanotoxicology ( IF 5 ) Pub Date : 2021-03-12 , DOI: 10.1080/17435390.2021.1894614
Bohu Pan 1 , Pravin R. Kaldhone 1 , Alexander W. Alund 1 , Hua Du 1 , Xiaoqing Guo 1 , Jian Yan 1 , Ying Chen 1 , Tong Zhou 2 , Timothy W. Robison 3 , Tao Chen 1
Affiliation  

Abstract

The increasing medical and food applications of silver nanoparticles (AgNPs) raise concerns about their safety, including the potential health consequences of human exposure. Previous studies found that AgNPs were negative in the Ames test due to both their microbicidal activity and the inability of nanoparticles to penetrate bacterial cell walls. Thus, the mutagenicity of AgNPs is still not completely clear, though they do induce chromosome damage, as suggested by many previous genotoxicity studies. In this study, whole-genome sequencing (WGS) was used to analyze the mutagenicity of AgNPs in mouse lymphoma cells expanded from single-cell clones. The cells were treated with AgNPs, 4-nitroquinolone-1-oxide (4-NQO) as the positive control, and vehicle controls. Both AgNPs and 4-NQO significantly increased mutation frequencies over their concurrent controls by 1.12-fold and 4.89-fold with mutation rates at 4-fold and 130-fold, respectively. AgNP-induced mutations mainly occurred at G:C sites with G:C > T:A transversions, G:C > A:T transitions, and deletions as the most commonly observed mutations. AgNPs also induced higher fold changes in tandem mutations. The results suggest that the WGS mutation assay conducted here can detect the low-level mutagenicity of AgNPs, providing substantial support for the use of the WGS method as a possible alternative assay with respect to the mutagenic assessment of nanomaterials.



中文翻译:

使用全基因组测序评估银纳米颗粒在小鼠淋巴瘤细胞中的致突变性

摘要

银纳米颗粒(AgNPs)在医疗和食品中的应用不断增加,引起人们对其安全性的担忧,包括人类暴露于健康的潜在后果。先前的研究发现,由于AgNPs的杀菌活性和纳米颗粒无法穿透细菌细胞壁,因此在Ames测试中阴性。因此,AgNPs的致突变性仍未完全清楚,尽管它们确实会引起染色体损伤,正如许多先前的遗传毒性研究所表明的那样。在这项研究中,全基因组测序(WGS)用于分析从单细胞克隆扩增而来的小鼠淋巴瘤细胞中AgNP的致突变性。用AgNP,4-硝基喹诺酮-1-氧化物(4-NQO)作为阳性对照和媒介物对照处理细胞。AgNP和4-NQO均比其同时存在的对照显着增加了1.12倍和4.89倍的突变频率,突变率分别为4倍和130倍。AgNP诱导的突变主要发生在G:C位点,其中G:C> T:A转变,G:C> A:T转变和缺失是最常见的突变。AgNPs还引起串联突变的更高倍数变化。结果表明,此处进行的WGS突变检测可以检测到AgNPs的低水平致突变性,为使用WGS方法作为对纳米材料诱变评估的可能替代检测方法提供了实质性支持。T转换和删除是最常见的突变。AgNPs还引起串联突变的更高倍数变化。结果表明,此处进行的WGS突变检测可以检测到AgNPs的低水平致突变性,为使用WGS方法作为对纳米材料诱变评估的可能替代检测方法提供了实质性支持。T转换和删除是最常见的突变。AgNPs还引起串联突变的更高倍数变化。结果表明,此处进行的WGS突变检测可以检测到AgNPs的低水平致突变性,为使用WGS方法作为对纳米材料诱变评估的可能替代检测方法提供了实质性支持。

更新日期:2021-04-05
down
wechat
bug