One representative of the order Trichoptera, namely the caddisfly Chaetopteryx villosa, was investigated along with the pygmy mole cricket Xya capensis which is a representative of the most basal superfamily of the caeliferan Orthoptera, that is, the Tridactyloidea. From both clades neuropeptides have not been biochemically characterized before this study. Here, members of the adipokinetic hormone family (AKHs) are sequenced via liquid chromatography (LC)‐ion trap mass spectrometry from methanolic extracts from the corpora cardiaca of respective species. The corpora cardiaca were dissected, methanolic extracts prepared, peptides separated by liquid chromatography (LC), and AKHs detected and sequenced by ion trap mass spectrometry. Both species investigated contain an octapeptide AKH: the trichopteran species has the peptide with the sequence pGlu‐Leu‐Thr‐Phe‐Thr‐Pro‐Ser‐Trp amide; the ambiguity of the isobaric amino acids Leu and Ile at position two was solved by comparing retention times on LC and by co‐elution with the synthetic Leu²‐form. This peptide is known as Aedae‐AKH and found in certain dipteran species and in an alderfly (Megaloptera). The tridactyloid species contains the peptide with the sequence pGlu‐Val‐Asn‐Phe‐Ser‐Pro‐Gly‐Trp amide which had first been identified in a member of the order Mantophasmatodea and is called Manto‐CC. Comparisons are made between the AKH complements of the sister groups Trichoptera and Lepidoptera and their possible relatedness and, on the other hand, between the AKH of X. capensis with those of closely related caeliferan superfamilies. The biology of the two studied species is used to speculate about a possible function of the elucidated hormones. Lastly, the use of a larval stage as starting material for structural neuropeptide information is discussed.

中文翻译：

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生化鉴定出的一个虫科昆虫（Trichoptera）和a鼠mole（直翅目：Caelifera：Tridactyloidea）中的神经肽

研究了鳞翅目目的一个代表，即鳞翅目Chaetopteryx villosa，以及py鼠mole的Xya capensis。它是caeliferan直翅目中最基础的超科，即Tridactyloidea的代表。在本研究之前，尚未从两个进化枝中对神经肽进行生物化学表征。在这里，脂肪动力学激素家族（AKHs）的成员通过液相色谱（LC）-离子阱质谱法从各自物种的胃体甲醇提取物中测序。解剖cardiac体，制备甲醇提取物，通过液相色谱法（LC）分离肽，并通过离子阱质谱法检测AKH并对其进行测序。被研究的两个物种都包含一个八肽AKH：三翅目物种具有的肽序列为pGlu-Leu-Thr-Phe-Thr-Pro-Ser-Trp酰胺；²表格。这种肽被称为Aedae-AKH，存在于某些双翅类物种和an蝇（Megaloptera）中。三倍体类物种包含具有pGlu-Val-Asn-Phe-Ser-Pro-Gly-Trp酰胺序列的肽，该肽首先在Mantophasmatodea的一个成员中被鉴定为Manto-CC。比较是姐妹团毛翅目和鳞翅目的AKH补充和他们可能的关联性，并在另一方面之间进行的AKH之间X.兔与这些密切相关caeliferan超家族。这两个研究物种的生物学被用来推测阐明激素的可能功能。最后，讨论了使用幼虫阶段作为结构神经肽信息的起始材料。