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Validation of resistance to root-knot nematode incorporated in peanut from the wild relative Arachis stenosperma
Agronomy Journal ( IF 2.1 ) Pub Date : 2021-03-15 , DOI: 10.1002/agj2.20654
Carolina Ballén‐Taborda 1 , Ye Chu 2 , Peggy Ozias‐Akins 1, 2 , Patricia Timper 3 , Scott A. Jackson 1, 4 , David J. Bertioli 1, 5 , Soraya C.M. Leal‐Bertioli 1, 6
Affiliation  

Peanut (Arachis hypogaea L.) root-knot nematode (PRKN) Meloidogyne arenaria is a very destructive pathogen to which most peanut cultivars are highly susceptible. Current peanut cultivars rely on a single locus for PRKN resistance incorporated from the wild relative A. cardenasii Krapov. & W.C. Greg., that could be overcome as a result of the emergence of new nematode populations. Thus, new sources of resistance are continually needed. A new and strong resistance has been found in the wild diploid relative, A. stenosperma Krapov. & W.C. Greg. Arachis stenosperma-derived quantitative trait loci (QTL) were described on chromosomes A02 and A09 and reduced nematode development by up to 98.2%. In order to validate these resistance segments, this study screened for PRKN resistance in BC2F1 lines and correlated with molecular genotypes. Here, six BC2F1s carrying chromosome introgressions in A02 and/or A09, showed strong resistance while one line was susceptible. Both phenotype and genotype data allowed us to validate and delineate the chromosomal segments in chromosomes A02 and A09 to ∼8.5Mbp and ∼6.5Mbp regions on the bottom of each, respectively. Within the QTL on A02 and top middle of both chromosomes A02 and A09 there are R-gene clusters, often implicated in pathogen resistance. We have provided validation of these key resistance QTL that can be used to inform breeding via marker selection and insights into the functional basis of resistance provided by the wild peanut relative A. stenosperma.

中文翻译:

野生近缘落花生对根结线虫抗性的验证

花生 ( Arachis hypogaea L.) 根结线虫 (PRKN)根结线虫是一种极具破坏性的病原体,大多数花生品种对它高度敏感。目前的花生栽培品种依赖于从野生近缘A. cardenasii Krapov合并的 PRKN 抗性的单一基因座。& WC Greg.,这可以通过新线虫种群的出现来克服。因此,不断需要新的抗性来源。在野生二倍体亲属A. stenosperma Krapov 中发现了一种新的强抗性。&WC 格雷格。花生在染色体 A02 和 A09 上描述了衍生的数量性状基因座 (QTL),并将线虫的发育减少了 98.2%。为了验证这些抗性片段,本研究筛选了 BC 2 F 1品系中的PRKN 抗性并与分子基因型相关联。这里,六个 BC 2 F 1在 A02 和/或 A09 中携带染色体渗入的 s 表现出很强的抗性,而其中一个品系易感。表型和基因型数据使我们能够验证和描绘染色体 A02 和 A09 中的染色体片段,分别为每个底部的~8.5Mbp 和~6.5Mbp 区域。在 A02 上的 QTL 以及 A02 和 A09 染色体的顶部中间,有 R 基因簇,通常与病原体抗性有关。我们提供了对这些关键抗性 QTL 的验证,这些 QTL 可用于通过标记选择和深入了解野生花生近缘A. stenosperma提供的抗性功能基础来为育种提供信息
更新日期:2021-03-15
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