Identification of the absorbed ingredients and metabolites in rats after an intravenous administration of Tanreqing injection using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry,Journal of Separation Science

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Identification of the absorbed ingredients and metabolites in rats after an intravenous administration of Tanreqing injection using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
Journal of Separation Science ( IF 3.1 ) Pub Date : 2021-03-14 , DOI: 10.1002/jssc.202000898
Like Xie _{1,

2} , Yangyang Wang _{2,

3} , Hao Yin _{2,

3} , Jiajia Li _{2,

3} , Zhou Xu _{2,

3} , Zhaolin Sun _{2,

3} , Fang Liu _{2,

3} , Xiaoli Zhang ₄ , Shaoyong Liu ₄ , Jianguo Sun ₁ , Xiaoting Tian _{2,

3} , Chenggang Huang _{2,

3}

Affiliation

The metabolic profiles of Tanreqing injection, which is a traditional Chinese medicine recommended for complementary administration to treat a novel coronavirus, have remained unclear, which inhibit the understanding of the effective chemical compounds of Tanreqing injection. In this study, a sensitive high-performance liquid chromatography quadrupole time-of-flight mass spectrometry method was used to identify the compounds and metabolites in various biosamples, including plasma, bile, liver, lung, kidney, urine, and feces, following the intravenous administration of Tanreqing injection in rats. A total of 89 compounds were characterized in the biosamples of Tanreqing injection-treated rats including 25 precursor constituents and 64 metabolites. Nine flavonoid compounds, twelve phenolic acids, and four iridoid glycosides were identified in the rats. Their metabolites were mainly produced by glucuronidation, deglucuronidation, glycosylation, deglycosylation, methylation, demethylation, N-heterocyclisation, sulphation, dehydroxylation, decarboxylation, dehydration, hydroxylation, and corresponding recombination reactions. This study was the first to comprehensively investigate the metabolic profile of Tanreqing injection and provides a scientific basis to further elucidate the pharmacodynamic material basis and therapeutic mechanism of Tanreqing injection.

更新日期：2021-03-14

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