Long-term cometabolic transformation of 1,1,1-trichlorethane (1,1,1-TCA) and 1,4-dioxane (1,4-D) was achieved using slow release compounds (SRCs) as growth substrates for pure cultures of Rhodococcus rhodochrous ATCC 21198 (ATCC strain 21198). Resting cell transformation tests showed 1,4-D transformation occurred without a lag phase for cells grown on 2-butanol, while an induction period of several hours was required for 1-butanol grown cells. These observations were consistent with activity-based labeling patterns for monooxygenase hydroxylase components and specific rates of tetrahydrofuran (THF) degradation. 1,1,1-TCA and 1,4-D degradation rates for alcohol-grown cells were slower than those for cells grown on gaseous alkanes such as isobutane. Batch metabolism and degradation tests were performed, in the presence of 1,1,1-TCA and 1,4-D, with the growth of ATCC strain 21198 on alcohols produced by the hydrolysis of orthosilicates. Three orthosilicates were tested: tetrabutylorthosilicate (TBOS), tetra-s-butylorthosilicate (T2BOS), and tetraisopropoxysilane (T2POS). The measured rates of alcohol release in poisoned controls depended on the orthosilicate structure with TBOS, which produced a 1° alcohol (1-butanol), hydrolyzing more rapidly than T2POS and T2BOS, that produced the 2° alcohols 2-butanol and 2-propanol, respectively. The orthosilicates were added as light non-aqueous phase liquids (LNAPLs) with ATCC strain 21198 and formed dispersed droplets when continuously mixed. Continuous rates of oxygen (O₂) consumption and carbon dioxide (CO₂) production confirmed alcohol metabolism by ATCC strain 21198 was occurring. The rates of metabolism (TBOS > T2POS > T2BOS) were consistent with the rates of alcohol release via abiotic hydrolysis. 1,4-D and 1,1,1-TCA were continuously transformed in successive additions by ATCC strain 21198 over 125 days, with the rates highly correlated with the rates of metabolism. The metabolism of the alcohols was not inhibited by acetylene, while transformation of 1,4-D and 1,1,1-TCA was inhibited by this gas. As acetylene is a potent inactivator of diverse bacterial monooxygenases, these results suggest that monooxygenase activity was required for the observed cometabolic transformations but not for alcohol utilization. Alcohol concentrations in the biologically active reactors were maintained below the levels of detection, indicating they were metabolized rapidly after being produced. Much lower rates of O₂ consumption were observed in the reactors containing T2BOS, which has benefits for in-situ bioremediation. The results illustrate the importance of the structure of the SRC when developing passive aerobic cometabolic treatment systems.

使用缓释化合物（SRC）作为纯培养物的生长底物可实现1,1,1-三氯乙烷（1,1,1-TCA）和1,4-二恶烷（1,4-D）的长期代谢性转化的玫瑰色红球ATCC 21198（ATCC菌株21198）。静止细胞转化试验表明，对于在2-丁醇上生长的细胞，没有滞后阶段发生了1,4-D转化，而1-丁醇生长的细胞需要几个小时的诱导期。这些观察结果与单加氧酶羟化酶组分的基于活性的标记模式和四氢呋喃（THF）降解的特定速率相一致。酒精生长的细胞的1,1,1-TCA和1,4-D降解速率比在气态烷烃（如异丁烷）上生长的细胞要慢。在1,1,1-TCA和1,4-D存在下，随着ATCC菌株21198在由原硅酸盐水解产生的醇上的生长，进行了批量代谢和降解测试。三个原硅酸盐进行了测试：tetrabutylorthosilicate（TBOS），四小号-正硅酸丁酯（T2BOS）和四异丙氧基硅烷（T2POS）。在中毒对照中测得的酒精释放速率取决于TBOS的原硅酸盐结构，TBOS生成1°醇（1-丁醇），水解速度比T2POS和T2BOS水解快，T2POS和T2BOS生成2°醇2-丁醇和2-丙醇， 分别。原硅酸盐以ATCC菌株21198的轻质非水相液体（LNAPLs）形式添加，并在连续混合时形成分散的液滴。连续消耗氧气（O ₂）和二氧化碳（CO _2））生产证实正在发生ATCC菌株21198的酒精代谢。代谢速率（TBOS> T2POS> T2BOS）与通过非生物水解释放的酒精速率一致。ATCC菌株21198在125天的连续添加过程中连续转化了1,4-D和1,1,1-TCA，其转化率与代谢速率高度相关。乙炔不会抑制醇类的代谢，而这种气体会抑制1,4-D和1,1,1-TCA的转化。由于乙炔是多种细菌单加氧酶的有效灭活剂，因此这些结果表明，所观察到的新陈代谢需要单加氧酶活性，而酒精利用则不需要。生物活性反应器中的酒精浓度保持在检测水平以下，表明它们在生产后迅速代谢。O的比率低得多在含有T2BOS的反应器中观察到_2种消耗量，这对原位生物修复具有好处。结果表明，在开发被动式有氧代谢治疗系统时，SRC结构的重要性。