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Infection of cucumber seedling roots by Pseudomonas amygdali pv. lachrymans following artificial substrate inoculation
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2021-03-15 , DOI: 10.1007/s10658-021-02251-6
Shengping Zhang , Xianglong Meng , Ying Cheng , Yanan Wang , Tongle Hu , Shutong Wang , Keqiang Cao

There are few studies of cucumber roots at the seedling stage infected by Pseudomonas amygdali pv. lachrymans (Pal). We used growth chamber assays to determine the infection of cucumber seedling roots by Pal following artificial substrate inoculation. Symptoms on cucumber roots, leaves, and stems were observed over time. We used real-time fluorescent quantitative PCR (qPCR) to obtain accurate numbers of Pal copies in infected roots, and then statistically assessed the dynamics of Pal copies in roots, stems, and leaves. Results indicated that Pal not only caused root decay, but also damaged the whole plant. Following root infection, the bacteria were transmitted through stems to the leaves. Cucumber seedlings in a moist planting substrate showed lodging, leaf chlorosis and wilt, and rotted roots. This study provides evidence that in moist substrate, Pal can infect cucumber roots and be transmitted throughout the host plant. In addition, the results also provide a foundation for further research on the transmission and proliferation of Pal in cucumber plants under field conditions.



中文翻译:

扁桃假单胞菌(Pseudomonas amygdali pv)感染黄瓜幼苗的根。人工底物接种后催泪弹

杏仁假单胞菌(pseudomonas amygdali pv)感染苗期黄瓜根的研究很少。拉赫里曼斯Pal)。在人工底物接种后,我们使用生长室测定法确定了Pal感染黄瓜幼苗根的能力。随着时间的推移,观察到黄瓜的根,叶和茎出现症状。我们使用实时荧光定量PCR(qPCR)获得感染根中Pal拷贝的准确数量,然后统计评估根,茎和叶中Pal拷贝的动态。结果表明,Pal不仅造成根腐烂,而且破坏了整个植物。根部感染后,细菌通过茎杆传播到叶片。潮湿的基质中的黄瓜幼苗表现出倒伏,叶绿化和枯萎以及根腐烂。这项研究提供的证据表明,在潮湿的基质中,Pal可以感染黄瓜根,并在整个寄主植物中传播。此外,该结果还为进一步研究田间条件下黄瓜植株中Pal的传播和增殖提供了基础。

更新日期:2021-03-15
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