Purpose

The purpose of this study was to assess eye movements during a multifocal ERG (mfERG) recording. This study evaluated the relationship between bivariate contour ellipse areas (BCEAs), mfERG amplitudes (Amps) and mfERG implicit times (ITs) with repeat testing and experienced subjects.

Methods

Thirty subjects were selected (15 experienced to ocular procedures and 15 novices). All were confirmed to have healthy retinas and at least 20/25 vision. MfERGs with a stimulus near 100% contrast and 4-min m-sequence were recorded on two different days using our common clinical technique, which did not constrain the head. VERIS with fundus monitoring system was used for recording with a Burian-Allen electrode. An external camera captured the fundus during each mfERG recording. The optic nerve head position was tracked in each video using a custom algorithm in order to determine BCEAs. Each subject performed one mfERG on two different days. MfERGs were analyzed for Amps and ITs for the fovea and whole eye.

Results

There was no correlation between the mfERG metrics and BCEAs with repeat testing. There were also no differences between the experienced and novice subjects for mfERG Amps, ITs or BCEAs. Eye movements between visits were highly correlated (multiple r = 0.67). BCEAs were larger during mfERGs (1.04 ± 0.8 deg²) than those observed in previous literature using brief viewing tasks (< 0.3 deg²). The proportion of time spent fixating within 1.0 and 2.0 degrees of the central hexagon was 68 and 93%, respectively.

Conclusions

This study is the first to evaluate the stability of the retina while recording a mfERG in healthy subjects and indicates that the center of fixation during a mfERG stays within the central hexagon. Eye stability during an initial recording is the best indicator of stability on the second recording. The amount of movement during these recordings did not seem to affect the mfERG Amps or ITs. These data suggest clinical confidence with mfERGs when recording novice patients.

中文翻译：

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mfERG 测试期间的眼底运动

目的

本研究的目的是评估多焦 ERG (mfERG) 记录期间的眼球运动。本研究通过重复测试和有经验的受试者评估了双变量轮廓椭圆区域 (BCEA)、mfERG 振幅 (Amps) 和 mfERG 隐式时间 (ITs) 之间的关系。

方法

选择了 30 名受试者（15 名经验丰富的眼科手术和 15 名新手）。所有人都被证实具有健康的视网膜和至少 20/25 的视力。使用我们常见的临床技术在两个不同的日子记录了具有接近 100% 对比度和 4 分钟 m 序列的刺激的 MfERG，这不会限制头部。带有眼底监测系统的 VERIS 用于使用 Burian-Allen 电极进行记录。在每次 mfERG 记录期间，外部摄像机捕获了眼底。使用自定义算法在每个视频中跟踪视神经乳头位置以确定 BCEA。每个受试者在两个不同的日子里进行了一次 mfERG。分析了 MfERG 的 Amps 和中央凹和整个眼睛的 IT。

结果

mfERG 指标和 BCEA 与重复测试之间没有相关性。对于 mfERG 放大器、IT 或 BCEA，经验丰富的受试者和新手受试者之间也没有差异。访问之间的眼球运动高度相关（倍数r  = 0.67）。mfERG 期间的 BCEAs (1.04 ± 0.8 deg ² ) 比使用简短查看任务 (< 0.3 deg ² )在以前的文献中观察到的大。固定在中心六边形 1.0 度和 2.0 度内的时间比例分别为 68% 和 93%。

结论

这项研究是第一个在健康受试者中记录 mfERG 时评估视网膜稳定性的研究，并表明 mfERG 期间的固定中心保持在中央六边形内。初次录制期间的眼睛稳定性是第二次录制时稳定性的最佳指标。这些录音期间的移动量似乎并未影响 mfERG 放大器或 IT。这些数据表明在记录新手患者时对 mfERG 的临床信心。