Anaerobically treated molasses-based distilleries spent wash is a major source of terrestrial and aquatic pollution due to the mixture of the various unknown organometallic compounds in discharged waste. The nature of pollutants present in effluent has to be characterized prior to the evaluation of their fate in the environment at various tropic levels. The absorption maxima (λ_max) were obtained at 295 nm of pollutants present in spent wash which indicated that melanoidins are a major colorant along with other organic compounds. Abundantly identified compounds with GC–MS in effluent were found: 1,2,4,5-tetrahydro-2-methyl-3H-2-benzazepine-3-one, 6-(trifluoromethyl)-1H-imidazole [4,5-c] pyridine, 4,6-di-t-butyl-1H, 3H-thieno[3,4-c] thiophene, butanoic acid, trimethylsilyl ester, silane, (dodecyloxy)trimethylsilyl ester, 4-styryl [2,2] paracyclophane, 4-(p-cumylphenoxy) phthalonitrile, hexadecanoic acid, trimethylsilyl ester, butyl ester, and squalene. Some of these compounds are known with mutagenic and androgenic properties. The toxicity test of post-methanated distillery effluent (PMDE) showed direct toxic effects on catfish (Heteropneustes fossilis) even at 1% (v/v) concentration and caused degeneration of primary and secondary lamellae of the gill and the epithelial layer. Further, higher concentrations between 5 and 10% of PMDE showed loss of primary and secondary lamellae of gills, and liver catalase activity was also increased dramatically in H. fossilis, indicating the generation of oxidative free radicals. Similarly, inhibition of α-amylase activities in germinating Zea mays L. (Maize) seeds was also noted at 10% PMDE. This study confirmed the environmental toxicity of effluent with freshwater fish and Zea mays seeds.

由于厌氧处理的以糖蜜为原料的蒸馏酒厂的洗净是陆地和水生污染的主要来源，这是由于排放的废物中混合了各种未知的有机金属化合物。在评估各种热带地区环境中的污染物命运之前，必须先确定废水中污染物的性质。最大吸收（λ_最大）是在废洗液中存在的295 nm污染物下获得的，这表明黑素和其他有机化合物是主要的着色剂。发现了使用气相色谱-质谱联用技术鉴定出的大量化合物：1,2,4,5-四氢-2-甲基-3H-2-苯并ze庚因-3-一，6-（三氟甲基）-1H-咪唑[4,5- c]吡啶，4,6-二叔丁基-1H，3H-噻吩并[3,4-c]噻吩，丁酸，三甲基甲硅烷基酯，硅烷，（十二烷氧基）三甲基甲硅烷基酯，4-苯乙烯基[2,2]对环烷，4-（对枯基苯氧基）邻苯二甲腈，十六烷酸，三甲基甲硅烷基酯，丁酯和角鲨烯。已知其中一些化合物具有诱变和雄激素特性。后甲烷化酒厂流出物（PMDE）的毒性试验表明上鲶鱼直接毒性作用（Heteropneustes fossilis）即使浓度为1％（v / v），也会导致and和上皮层的初级和次级片状细胞变性。此外，PMDE的5和10％之间的较高浓度显示鳃的原发性和继发性薄片的损失，和肝脏过氧化氢酶的活性也在急剧增加H. fossilis，表明氧化自由基的产生。同样，抑制玉米发芽中的α-淀粉酶活性。（玉米）种子的PMDE含量也为10％。这项研究证实了淡水鱼和玉米种子对废水的环境毒性。