当前位置: X-MOL 学术J. Mol. Neurosci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
An 8-Week Administration of Bifidobacterium bifidum and Lactobacillus plantarum Combined with Exercise Training Alleviates Neurotoxicity of Aβ and Spatial Learning via Acetylcholine in Alzheimer Rat Model
Journal of Molecular Neuroscience ( IF 3.1 ) Pub Date : 2021-03-14 , DOI: 10.1007/s12031-021-01812-y
Samaneh Shamsipour 1 , Gholamreza Sharifi 1 , Farzaneh Taghian 1
Affiliation  

This study aimed to determine the effects of 8 weeks of an administration of Bifidobacterium bifidum and Lactobacillus plantarum combined with exercise training on neurotoxicity of Aβ, spatial learning, acetylcholine (ACH), and vascular endothelial growth factor (VEGF) in Alzheimer rats. Twenty-five Wistar rats were randomly divided into 5 groups (n = 5 in each): (1) healthy control (control), (2) Alzheimer disease (AD), (3) AD with treadmill exercise (AD + Exe), (4) AD with probiotic (combined administration of Bifidobacterium bifidum and Lactobacillus plantarum) treatment (AD + Pro), and (5) AD with treadmill exercise and probiotic treatment (AD + Exe + Pro). AD was induced by intra-cerebroventricular injection of Aβ1-42 peptide. Then, the training groups exercised on treadmill for 8 weeks, 5 days per weeks. The rats were treated daily with probiotic supplements via gavage for 8 weeks. The Morris water maze (MWM) test was administered to measure spatial learning. Then, the animals were sacrificed and Vegf and ACH were analyzed using the qPCR and immunohistochemistry (IHC) methods, respectively. Results showed that the β-amyloid plaques were significantly increased in the brains of the AD group compared with the control group (p < 0.001). The combined use of probiotics and exercise training significantly increased the time spent in the target quadrant after removing the platform, compared with the AD group in the Morris water maze test (p < 0.001). Crystal violet analysis showed that sole (p < 0.01) and combined exercise training and probiotic supplementation (p < 0.001) significantly reduced the number of dead cells in the brains of rats compared with the AD group. AD significantly decreased Vegf mRNA and ACH in the CA1 area of the hippocampus (p < 0.001). However, mono and combined therapy (exercise and probiotics) significantly increased ACH in the rats’ brain compared with the AD group. Overall, 8 weeks of an administration of Bifidobacterium bifidum and Lactobacillus plantarum combined with exercise training can improve spatial learning impairment in the AD rats. Exercise and probiotics seem to offer potential benefits to AD patients by upregulating ACH.



中文翻译:

为期 8 周的双歧杆菌和植物乳杆菌给药联合运动训练可减轻阿尔茨海默病大鼠模型中 Aβ 的神经毒性和乙酰胆碱的空间学习

本研究旨在确定给予双歧杆菌植物乳杆菌8 周联合运动训练对阿尔茨海默病大鼠 Aβ、空间学习、乙酰胆碱 (ACH) 和血管内皮生长因子 (VEGF) 的神经毒性的影响。将 25 只 Wistar 大鼠随机分为 5 组(每组n  = 5):(1)健康对照(对照),(2)阿尔茨海默病(AD),(3)AD 与跑步机运动(AD + Exe), (4)AD加益生菌(双歧双歧杆菌植物乳杆菌联合给药)) 治疗 (AD + Pro),和 (5) AD 与跑步机运动和益生菌治疗 (AD + Exe + Pro)。AD通过脑室内注射Aβ1-42肽诱导。然后,训练组在跑步机上锻炼 8 周,每周 5 天。每天通过管饲法对大鼠进行益生菌补充剂治疗 8 周。莫里斯水迷宫 (MWM) 测试用于测量空间学习。然后,处死动物,分别使用 qPCR 和免疫组织化学 (IHC) 方法分析Vegf和 ACH。结果显示,与对照组相比,AD组脑内β-淀粉样斑块明显增多(p < 0.001)。在 Morris 水迷宫试验中,与 AD 组相比,益生菌联合使用运动训练显着增加了移除平台后在目标象限停留的时间(p  < 0.001)。结晶紫分析表明,与AD组相比,单独(p  < 0.01)和联合运动训练和益生菌补充(p  < 0.001)显着减少了大鼠大脑中的死细胞数量。AD 显着降低海马 CA1 区的Vegf mRNA 和 ACH( p  < 0.001)。然而,与 AD 组相比,单一和联合治疗(运动和益生菌)显着增加了大鼠大脑中的 ACH。总体而言,8周的管理双歧杆菌植物乳杆菌联合运动训练可改善AD大鼠空间学习障碍。运动和益生菌似乎通过上调 ACH 为 AD 患者提供了潜在的益处。

更新日期:2021-03-15
down
wechat
bug