Abstract

A new, nonglycosylated, serine proteinase capable of hydrolyzing collagen, elastin, and fibrin was isolated from a complex preparation of proteins formed by the micromycete A. ustus 1 under solid-state fermentation. The enzyme had a molecular weight of 33 kDa and a pI of 4.6. The pH-optimum (6.0) and temperature optimum (41°C) of the work of this proteinase were also determined.

中文翻译：

---

微霉菌曲霉1胞外蛋白酶的性质及其在纤维蛋白水解过程中的高活性

摘要

在固态发酵条件下，从由微霉菌A. ustus 1形成的蛋白质的复杂制剂中分离出了一种能够水解胶原蛋白，弹性蛋白和纤维蛋白的新型非糖基化丝氨酸蛋白酶。该酶的分子量为33 kDa，pI为4.6。还确定了该蛋白酶工作的最适pH（6.0）和最适温度（41℃）。