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Characterization and Quantification of Luteolin-Metal Complexes in Aqueous Extract of Lonicerae Japonicae Flos and Huangshan Wild Chrysanthemum
International Journal of Analytical Chemistry ( IF 1.8 ) Pub Date : 2021-03-12 , DOI: 10.1155/2021/6677437
Weilan Cai 1 , Yunhao Xiong 1 , Manman Han 1 , Zhimin Li 1 , Liang Peng 1 , Hongyi Zhang 1 , Qin Zou 1 , Lin Wu 1 , Qingling Ye 1 , Linfeng Liao 1
Affiliation  

Luteolin is a flavonoid compound widely found in vegetables, fruits, and medicinal plants. In this study, the reaction conditions for luteolin and five metal ions (Ca2+, Mg2+, Zn2+, Fe3+, and Cu2+) to form complexes in hot water were optimized, which was at a molar ratio of 1 : 1 for luteolin and metal ions at 90°C in a volume of 20 mL for 2 h, and the ability of luteolin to form complexes with Cu2+ was the strongest. The DPPH scavenging test showed that luteolin exerted a dose-dependent effect on the clearance of free radicals; luteolin-Cu2+ complexes and luteolin-Fe3+ complexes accentuated the clearance of free radicals. Furthermore, we used high performance liquid chromatography (HPLC) to analyze luteolin in samples from two medicinal plants, obtained from the dissolution of aqueous extracts in two different solvents. The results showed that the peak areas for luteolin in the samples dissolved in 20% formic acid-methanol were significantly larger than those from the samples dissolved in methanol alone, with increases in the peak area being 135.6% (Lonicerae Japonicae Flos), and 161.16% (Huangshan wild chrysanthemum). The aforementioned results indicate that complexes formed from organic compounds and metal ions are present in the decoction of a plant.

中文翻译:

金银花和黄山野菊花水提物中木犀草素-金属配合物的表征与定量

木犀草素是一种类黄酮化合物,广泛存在于蔬菜,水果和药用植物中。本研究优化了木犀草素与5种金属离子(Ca 2 +,Mg 2 +,Zn 2 +,Fe 3+和Cu 2+)在热水中形成配合物的反应条件,且该反应条件为摩尔比在90°C下以20:1的体积将木犀草素和金属离子按1:1的比例混合2小时,木犀草素与Cu 2+形成络合物的能力最强。DPPH清除试验表明,木犀草素对清除自由基具有剂量依赖性。木犀草素-Cu 2+络合物和木犀草素-Fe 3+配合物加重了自由基的清除。此外,我们使用高效液相色谱(HPLC)分析了两种药用植物样品中的木犀草素,这些样品是将水提物溶解在两种不同溶剂中而获得的。结果表明,溶于20%甲酸-甲醇的样品中木犀草素的峰面积明显大于仅溶于甲醇的样品中木犀草素的峰面积,其峰面积增加了135.6%(忍冬科植物忍冬)和161.16。 %(黄山野菊花)。前述结果表明在植物的煎剂中存在由有机化合物和金属离子形成的络合物。
更新日期:2021-03-12
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