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Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
npj Biofilms and Microbiomes ( IF 9.2 ) Pub Date : 2021-03-11 , DOI: 10.1038/s41522-021-00192-w
Yu-chao Tseng, Hsin-yi Yang, Wei-ting Lin, Chia-bin Chang, Hsiu-chuan Chien, Hon-pin Wang, Chun-ming Chen, Jann-tay Wang, Chin Li, Shu-fen Wu, Song-chou Hsieh

Salivary gland epithelial cells (SGECs) have been implicated in the pathogenesis of Sjögren’s syndrome due to aberrant antigen-presentation function. This study examined the hypothesis that oral dysbiosis modulates the antigen-presentation function of SGECs, which regulates CD4 T cell proliferation in primary Sjögren’s syndrome (pSS). Saliva samples from 8 pSS patients and 16 healthy subjects were analyzed for bacterial 16S ribosomal DNA. As a result, 39 differentially abundant taxa were identified. Among them, the phylum Proteobacteria comprised 21 taxa, and this phylum was mostly enriched in the healthy controls. The proteobacterium Haemophilus parainfluenzae was enriched in the healthy controls, with the greatest effect size at the species level. Treatment of A253 cells in vitro with H. parainfluenzae upregulated PD-L1 expression, and H. parainfluenzae-pretreated A253 cells suppressed CD4 T cell proliferation. The suppression was partially reversed by PD-L1 blockade. Among low-grade xerostomia patients, salivary abundance of H. parainfluenzae decreased in pSS patients compared to that in non-pSS sicca patients. Our findings suggest that H. parainfluenzae may be an immunomodulatory commensal bacterium in pSS.



中文翻译:

干燥综合征中的唾液生态失调和唾液腺上皮细胞的共生介导的免疫调节作用

由于异常的抗原呈递功能,唾液腺上皮细胞 (SGEC) 与干燥综合征的发病机制有关。这项研究检验了口腔生态失调调节 SGECs 的抗原呈递功能的假设,SGECs 调节原发性干燥综合征 (pSS) 中的 CD4 T 细胞增殖。对 8 名 pSS 患者和 16 名健康受试者的唾液样本进行了细菌16S核糖体 DNA 分析。结果,确定了 39 个差异丰富的分类群。其中,Proteobacteria 门包含 21 个分类群,该门在健康对照中最为丰富。该变形菌副流感嗜血杆菌在健康对照中富集,在物种水平上具有最大的影响大小。用流感嗜血杆菌体外处理 A253 细胞上调 PD-L1 表达,副流感嗜血杆菌预处理的 A253 细胞抑制 CD4 T 细胞增殖。这种抑制被 PD-L1 阻断部分逆转。在低度口干症患者中,与非 pSS 干燥症患者相比,pSS 患者的副流感嗜血杆菌唾液丰度降低。我们的研究结果表明,副流感嗜血杆菌可能是 pSS 中的一种免疫调节共生菌。

更新日期:2021-03-11
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