The present study proposes a simple and rapid protocol to isolate DNA from marine algae of the genus Sargassum and Gracilaria. To identify the easy, fast and low cost method, four methods of DNA isolation viz., The Robertson Labs methods, STE Buffer method, PureFast-Plant Genomic DNA purification kit by Helini Biomolecules, Chennai and DNA Isolation by Heat Lysis Method were chosen for the present study. To optimize the DNA isolation methods, the thalli of Sargassum plagiophyllum C. Ag. and Gracilaria salicornia C.Ag. were employed as a source of DNA. The DNA isolated based on three genomic DNA extraction protocols and by heat lysis method varied with reference to method employed for the isolation. By heat lysis method, the DNA of S. plagiophyllum and G. salicornia was isolated and simultaneously amplified (400 bp) using 23S rRNA gene primers. With these results, the DNA of G. salicornia, G. edulis, G. corticata, G. fergusonii, G. verrucosa, S. aquifolium, S. plagiophyllum, S. polycystum, S. tenerrimum and S. swartzii were isolated and amplified using heat lysis method. The heat lysis method is thereby proven to be efficient, rapid, simple, cost effective and requires only a small fraction of the sample. The heat lysis method can be a successful replacement for the conventional DNA isolation methods, when used specifically for DNA barcoding technique targetting organelle DNA.

本研究提出了一种简单且快速的方案，可以从Sargassum和Gracilaria属的海藻中分离DNA 。为了鉴定简便，快速和低成本的方法，选择了四种DNA分离方法，即Robertson Labs方法，STE Buffer方法，Helini Biomolecules的PureFast-Plant Genomic DNA纯化试剂盒，Chennai和热裂解法进行DNA分离。本研究。为了优化DNA分离方法，Sargassum plagiophyllum C. Ag。的thalli。和河南水杨C.Ag. 被用作DNA的来源。基于三种基因组DNA提取方案并通过热裂解法分离的DNA随分离方法的不同而变化。通过热裂解法，分离了S. plagiophyllum和G. salicornia，并使用23S rRNA基因引物同时扩增（400 bp）。与这些结果，的DNA G.海蓬子，G.贻贝，G. corticata，G. fergusonii，G.疣状，S.尖头叶，S. plagiophyllum，S. polycystum，S. tenerrimum和S. swartzii进行分离和扩增采用热裂解法。由此证明了热裂解方法是有效，快速，简单，成本有效的，并且仅需要样品的一小部分。当热裂解方法专门用于靶向细胞器DNA的DNA条形码技术时，可以成功替代传统的DNA分离方法。