ABSTRACT

This study aimed to investigate the chemical composition and antifungal activities of essential oil (EO) of Teucrium luteum subsp. flavovirens. The analysis of EO using gas chromatography (GC-FID) and gas chromatography-mass spectroscopy (GC-MS) allowed the identification of 63 compounds, accounting for 89.9% of the total oil. The major constituents were E-nerolidol (31.0%), β-pinene (6.9%), elemol (5.0%), and α-pinene (4.5%). The antifungal activities of EO in liquid and vapor-phase were evaluated in vitro against three apple pathogenic fungi using poisoned food (PF) and volatile activity (VA) techniques. The results indicated that the EO inhibited significantly the mycelial growth of all strains tested (p < .05). The minimum inhibitory concentration (MIC) against Alternaria sp. was 2 µL/mL in VA assay, whereas >2 µL/mL in PF technique for all strains. Fungal spore production, in vapor phase, was completely inhibited at 2 µL/mL for three phytopathogens. The antifungal activities of T. luteum subsp. flavovirens from the Southeast of Morocco are reported for the first time.

摘要

这项研究旨在调查黄精亚种的精油（EO）的化学成分和抗真菌活性。黄病毒。使用气相色谱法（GC-FID）和气相色谱质谱法（GC-MS）对EO进行分析，可以鉴定出63种化合物，占总油量的89.9％。主要成分为E-神经氨酸（31.0％），β-pine烯（6.9％），elemol（5.0％）和α-pine烯（4.5％）。使用有毒食品（PF）和挥发性活性（VA）技术，体外评估了EO在液相和气相中的抗真菌活性，针对三种苹果病原真菌。结果表明，EO显着抑制了所有测试菌株的菌丝生长（p<.05）。对链格孢菌的最低抑制浓度（MIC）。在VA分析中为2 µL / mL，而在所有菌株中PF技术为> 2 µL / mL。三种植物病原体以2 µL / mL的浓度完全抑制了气相中真菌孢子的产生。黄萎病菌亚种的抗真菌活性。首次报道了来自摩洛哥东南部的黄病毒。