Abstract

In this work, bionanocomposite membranes of hydroxyapatite nanoparticles (nHA) embedded in chitosan (CHI) or carboxymethyl-chitosan (CMC) matrices were chemically crosslinked with epichlorohydrin (ECH) for tuning their physicochemical and biological properties aiming at bone tissue regeneration. The choice of CHI or CMC biopolymer, as well as the presence of ECH crosslinker, affected the nucleation and growth processes of nHA producing average diameters varying from 93 nm to 589 nm and altered the relative stability of the membranes against degradation. In vitro analysis by MTT using SAOS and HEK 293 T cell lineages evidenced preliminary cytocompatibility for the biocomposites investigated. Moreover, in vivo analyses using the Wistar rat model demonstrated that the crosslinking enhanced bone repair processes in up to 55% and 22% compared to the control and non-crosslinked biocomposites, respectively.

摘要

在这项工作中，嵌入壳聚糖 (CHI) 或羧甲基壳聚糖 (CMC) 基质中的羟基磷灰石纳米颗粒 (nHA) 的生物复合膜与表氯醇 (ECH) 进行化学交联，以调整其物理化学和生物学特性，以促进骨组织再生。CHI 或 CMC 生物聚合物的选择以及 ECH 交联剂的存在影响了 nHA 的成核和生长过程，产生了从 93 nm 到 589 nm 的平均直径，并改变了膜对降解的相对稳定性。使用 SAOS 和 HEK 293 T 细胞谱系通过 MTT 进行的体外分析证明了所研究的生物复合材料的初步细胞相容性。此外，在体内使用 Wistar 大鼠模型进行的分析表明，与对照和非交联的生物复合材料相比，交联增强的骨修复过程分别高达 55% 和 22%。