Abstract

Growth performance and meat quality are important traits for pig production. The aim of the present study was to investigate the molecular mechanisms underlying growth performance and meat quality, and to identify novel target molecules for predicting the growth performance and meat quality. The differentially expressed genes (DEGs) in Diannan small ears pigs (DSP) and Landrace pigs (LP) were assessed by RNA-sequencing analyzing technology. A total of 339 DEGs were obtained between DSP and LP. 146 DEGs were upregulated in LP compared with DSP and 193 DEGs were upregulated in DSP compared with LP. The DEGs were significantly enriched in 26 GO and 3 KEGG pathways. The protein–protein interaction (PPI) network with 201 nodes and 382 edges was constructed and 5 modules were extracted from the entire network. The identified upregulated expression of genes involved in glycolysis and myogenesis as well as extracellular matrix may be associated with fast body and muscle deposition rates in LP. Increased expression of genes involved in PPAR signaling pathway and fatty acid metabolism as well as oxidative phosphate processes could be related to the intramuscular fat deposition and meat quality in DSP. The present study may provide an improved understanding of the growth performance and meat quality.

摘要

生长性能和肉质是生猪生产的重要特性。本研究的目的是研究生长性能和肉质的分子机制，并确定用于预测生长性能和肉质的新目标分子。采用RNA测序分析技术对滇南小耳猪(DSP)和长白猪(LP)的差异表达基因(DEGs)进行了评估。DSP 和 LP 之间总共获得了 339 个 DEG。与 DSP 相比，LP 中有 146 个 DEG 上调，与 LP 相比，DSP 中有 193 个 DEG 上调。DEGs 在 26 个 GO 和 3 个 KEGG 通路中显着富集。构建了具有 201 个节点和 382 个边的蛋白质-蛋白质相互作用 (PPI) 网络，并从整个网络中提取了 5 个模块。已确定的参与糖酵解和肌生成以及细胞外基质的基因表达上调可能与 LP 中快速的身体和肌肉沉积率有关。参与 PPAR 信号通路和脂肪酸代谢以及氧化磷酸盐过程的基因表达增加可能与 DSP 中的肌肉内脂肪沉积和肉质有关。本研究可能会更好地了解生长性能和肉质。