The effects of viscous media with glycerol and sucrose (10–40%) on the kinetics of the bacterial bioluminescent reaction have been investigated by stopped-flow technique. Increment of quantum yield in media with 10% of both osmolytes was shown. Higher concentrations of glycerol, up to 30–40%, were found to reduce the efficiency of the reaction, while this effect was not observed in the media with sucrose. The molecular dynamics simulation was used to study the structure of bacterial luciferase surrounded by either water molecules solely or by mixture of water with various numbers of glycerol/sucrose molecules. It was found that both cosolvents at studied concentrations did not cause a significant change in conformation of bacterial luciferase. The calculated root-mean-square fluctuation for C_α-atoms of bacterial luciferase α-subunit indicated that the higher flexibility of the enzyme mobile loop could be responsible for increment of quantum yield in the presence of 10% of both osmolytes. The active site of bacterial luciferase was found to be accessible for glycerol molecules while sucrose did not enter catalytic gorge. Moreover, at 30 and 40% concentration the glycerol molecules were found to locate in the active site of bacterial luciferase throughout the whole simulation time.

用停止流技术研究了甘油和蔗糖（10-40％）的粘性介质对细菌生物发光反应动力学的影响。显示了两种渗透液均含有10％的培养基中量子产率的增加。发现较高浓度的甘油（高达30–40％）会降低反应效率，而在含蔗糖的培养基中未观察到这种效果。分子动力学模拟被用来研究细菌荧光素酶的结构，该结构被水分子单独包围或被水与各种数量的甘油/蔗糖分子混合而被包围。发现在所研究的浓度下两种助溶剂都不会引起细菌荧光素酶构象的显着变化。所计算的根均方波动对C _α细菌萤光素酶α-亚基的原子表明，在两种渗透液均存在10％的情况下，酶移动环的较高灵活性可能是导致量子产率增加的原因。发现细菌荧光素酶的活性位点对于甘油分子是可接近的，而蔗糖没有进入催化峡谷。此外，在整个模拟时间内，发现浓度为30％和40％的甘油分子位于细菌荧光素酶的活性位点。