The removal of textile dye using the biosorption technique is important for improving water quality and avoiding the harmful effects of the traditional approach for removing wastewater on human health. Lichens are symbiotic organisms that have great potential for decolorization and degradation of textile dye. The present study evaluates Acid Red P-2BX dye decolorization by living lichen Evernia prunastri at the pH range of 2–10. Dye decolorization rates at pH 2 were 84.62%, 87.5% and 88.54% at 25, 50 and 100 mg/L of initial dye concentrations, respectively. The pigment analysis and mean intensity of chlorophyll autofluorescence results indicated that pH could be the main factor affecting lichen physiology and thalli decolorization capacity. Fourier transform infrared spectroscopy was used as a high-throughput method for understanding biomolecular changes in thalli. The lipid/amide I and carbohydrate/amide I ratio increased at pH 2. The present study also indicated, for the first time, that decolorization rate is related to altering lichen carbohydrate and especially lipid metabolism in the E. prunastri thalli. Thus, we demonstrated that the lichen E. prunastri could be a good candidate for use as a biosorbent.

使用生物吸附技术去除纺织品染料对于改善水质和避免传统的废水去除方法对人体健康的有害影响非常重要。地衣是共生生物，对纺织品染料的脱色和降解具有巨大潜力。本研究评估了生活性地衣Evernia prunastri对酸性红P-2BX染料的脱色在2-10的pH范围内。在25、50和100 mg / L的初始染料浓度下，pH 2下的染料脱色率分别为84.62％，87.5％和88.54％。色素分析和叶绿素自发荧光的平均强度表明，pH可能是影响地衣生理和塔利脱色能力的主要因素。傅里叶变换红外光谱法是一种高通量方法，用于了解塔利生物分子的变化。在pH 2下，脂质/酰胺I和碳水化合物/酰胺I的比率增加。本研究还首次表明，脱色率与改变地衣中碳水化合物的含量有关，特别是与苦菜中的脂质代谢有关。因此，我们证明了地衣E. prunastri 可能是用作生物吸附剂的良好候选者。