Ticks are obligate blood-sucking parasitic arthropods. When sucking the blood of hosts, they can also transmit a variety of pathogens to hosts that severely endanger the health of humans and animals. The spermatheca is an organ for the storage and protection of sperm and an important component of the reproductive system of female ticks. The spermatheca content changes dramatically over time after copulation. In particular, some proteins and polypeptide substances can influence the physiological functions of female ticks and promote blood feeding and egg laying by female ticks. To investigate the molecular mechanisms underlying the productive process of Haemaphysalis longicornis, data-independent acquisition (DIA) quantitative proteomics technology was used to perform in-depth research of the dynamic changes in all proteins in the spermatheca of ticks within a short time after copulation to look for key proteins in the spermatheca contents after copulation that affect the reproduction of female ticks in order to provide meaningful information for the comprehensive prevention and control of ticks.

cks虫是专性吸血寄生节肢动物。当吸取宿主的血液时，它们还可以将多种病原体传播到宿主，从而严重危害人类和动物的健康。精子是精子的储存和保护器官，是雌性tick的生殖系统的重要组成部分。交配后，精子的含量随时间变化很大。特别地，某些蛋白质和多肽物质可以影响雌性tick的生理功能，并促进雌性blood的采血和产卵。调查血红蛋白生产过程的分子机制，采用了数据独立获取（DIA）定量蛋白质组学技术，对交后短时间内s的所有蛋白质的动态变化进行了深入研究，以寻找影响交配后精子内容物中关键影响的蛋白质。雌性s的繁殖，以便为全面预防和控制s提供有意义的信息。