Bulletin of Environmental Contamination and Toxicology ( IF 2.7 ) Pub Date : 2021-03-11 , DOI: 10.1007/s00128-021-03164-0 Maryam Ebrahimzadeh , Behrooz Heidari , Fatemeh Nazarhaghighi , Abdolmajid Valipour
The aquatic environment is constantly exposed to chemical pollutants from agriculture and the urban environment. In this study, the effects of Bisphenol A, Naphthalene, and Butachlor on metabolic and antioxidant enzymes of goldfish were investigated during subacute exposure. The samples (n = 144, 100.97 ± 31.47 g) were distributed randomly in 12 glass aquaria (70 L) with a stocking density of 12 fish for each. Each aquarium was exposed to one of each pollutant (Bisphenol A with 500 μg/L, Butachlor with 0.28 μL/L and Naphthalene with 200 μg/L) separately, while the control group remained without contaminants for the entire 5 weeks of the experiment. The result showed that the activity of AST (Aspartate transaminase), ALT (Alanine transaminase), ALP (alkaline phosphatases), and POD (Guaiacol Peroxidase) significantly altered in contaminants treatments. The POD level, an antioxidant enzyme, showed a significant increase, especially on the 14th day of exposure to Bisphenol A (p < 0.05). In conclusion, these enzymes, as a biomarker, can be useful in environmental biomonitoring of aquatic ecosystems.
中文翻译:
金鱼(assi鱼)在亚急性接触有机污染物期间的生理反应。
水生环境不断暴露于农业和城市环境中的化学污染物。在这项研究中,研究了双酚A,萘和丁草胺对亚急性暴露过程中金鱼代谢和抗氧化酶的影响。将样本(n = 144,100.97±31.47 g)随机分配到12个玻璃水族箱(70 L)中,每个鱼类的放养密度为12条鱼。每个水族箱分别暴露于一种污染物(双酚A为500μg/ L,丁草胺为0.28μL/ L和萘为200μg/ L),而对照组在整个实验的5周中均保持着无污染物状态。结果表明,AST(天冬氨酸转氨酶),ALT(丙氨酸转氨酶),ALP(碱性磷酸酶),和POD(愈创木酚过氧化物酶)在污染物处理中发生了显着变化。POD是一种抗氧化酶,其含量显着增加,尤其是在暴露于双酚A(第14天)的第14天p <0.05)。总之,这些酶作为生物标志物,可用于水生生态系统的环境生物监测。