Adaptive optics scanning light ophthalmoscopy (AOSLO) allows non-invasive visualization of the living human eye at the microscopic scale; but even with correction of the ocular wavefront aberrations over a large pupil, the smallest cells in the photoreceptor mosaic cannot always be resolved. Here, we synergistically combine annular pupil illumination with sub-Airy disk confocal detection to demonstrate a 33% improvement in transverse resolution (from 2.36 to 1.58 µm) and a 13% axial resolution enhancement (from 37 to 32 µm), an important step towards the study of the complete photoreceptor mosaic in heath and disease. Interestingly, annular pupil illumination also enhanced the visualization of the photoreceptor mosaic in non-confocal detection schemes such as split detection AOSLO, providing a strategy for enhanced multimodal imaging of the cone and rod photoreceptor mosaic.

中文翻译：

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利用环形瞳孔照明和亚艾里检测对人眼光感受器进行体内亚衍射自适应光学成像

自适应光学扫描光检眼镜 (AOSLO) 可以在微观尺度上对活体人眼进行非侵入性可视化；但即使校正了大瞳孔上的眼部波前像差，也无法始终分辨出感光细胞镶嵌中的最小细胞。在这里，我们将环形光瞳照明与亚艾里斑共焦检测相结合，证明横向分辨率提高了 33%（从 2.36 到 1.58 µm），轴向分辨率提高了 13%（从 37 到 32 µm），这是朝着实现这一目标迈出的重要一步。研究健康和疾病中完整的光感受器镶嵌体。有趣的是，环形光瞳照明还增强了非共焦检测方案（例如分裂检测 AOSLO）中光感受器马赛克的可视化，为增强视锥和杆光感受器马赛克的多模态成像提供了策略。