Abstract—

Accurate strain identification within the Pantoea genus is difficult due to homologous recombination, which may affect the species boundaries. An integrated approach is presently the most effective one in determining the species of bacteria. Biochemical identification using the API20E system, phylogenetic analysis of the 16S rRNA gene sequences, and MLSA analysis based on partial sequences of the fusA, pyrG, leuS, gyrB, and rpoB genes showed that the soil phytate-hydrolyzing isolates belonged to the genus Pantoea, specifically to the species Pantoea brenneri. It was also established that phytate-hydrolyzing activity of the strains was accompanied by their ability to fix atmospheric nitrogen.

中文翻译：

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基于表型特征和多基因座序列分析（MLSA）的泛植酸水解根瘤菌的鉴定

摘要—

由于同源重组，在泛菌属中难以准确鉴定菌株，这可能会影响物种边界。目前，一种综合方法是确定细菌种类的最有效方法。使用API​​20E系统进行的生化鉴定，16S rRNA基因序列的系统发育分析以及基于fusA，pyrG，leuS，gyrB和rpoB基因的部分序列的MLSA分析表明，水解植酸盐的分离株属于泛菌属，专门针对该物种Pantoea brenneri。还确定了菌株的肌醇六磷酸水解活性伴随着它们固定大气氮的能力。