Abstract

Ticks are economically important obligatory blood feeding arthropods that have a pivotal role in transmission of infection. The present study was conducted in ixodid ticks collected from four districts of coastal Odisha, India to investigate the prevalence of Theileria annulata. Adult semi engorged Hyalomma anatolicum ticks (n = 178) were dissected, the salivary gland was isolated and DNA was extracted. A nested PCR targeting the Tams1 gene of T. annulata, utilizing two sets of primers (N516F, N517R, and Ta14136iF, Ta249R) was utilized for detection of the parasite. The PCR products were then sequenced and subjected to BLAST analysis, alignment, and phylogenetic study. Two sequences deposited in GenBank were assigned Accession No MH477290.1 and Accession No MH477291.1. The molecular investigation of T. annulata revealed an overall prevalence of 14.6% in tick vectors, and nested PCR was found to have significant (p < 0.05) higher results than primary PCR. A significant higher presence (p < 0.05) was recorded in female ticks compared with male ticks. This is the first report of detection of the parasite in tick vectors in the state of Odisha.

摘要

蜱虫是经济上重要的强制性血液喂养节肢动物，在感染传播中起着关键作用。本研究是在从印度奥里萨邦沿海的四个地区收集的硬蜱中进行的，以调查环纹泰勒虫的流行情况。解剖成年半肿胀的Hyalomma anatolicum蜱（n  = 178），分离唾液腺并提取 DNA。针对T. annulata的 Tams1 基因的嵌套 PCR，利用两组引物（N516F、N517R 和 Ta14136iF、Ta249R）用于检测寄生虫。然后对 PCR 产物进行测序并进行 BLAST 分析、比对和系统发育研究。保藏在 GenBank 中的两个序列指定登录号 MH477290.1 和登录号 MH477291.1。T. annulata的分子研究显示蜱虫载体的总体流行率为 14.6%，并且发现巢式 PCR 的结果显着 ( p  < 0.05) 高于初级 PCR。 与雄性蜱虫相比，雌性蜱虫的存在显着更高 ( p < 0.05)。这是在奥里萨邦检测到蜱虫寄生虫的第一份报告。