Salmonella enterica is an important foodborne pathogen. Its rapid and reliable detection in food is critical for the effective prevention of the outbreak of foodborne diseases. Here, we report a novel method for the rapid, specific, label-free, and real-time detection of S. enterica with biolayer interferometry (BLI) using an antibody as the receptor. The limit of detection for S. enterica in buffer was 1.6 × 10⁵ CFU/ml at a binding time of 300 s. This method enabled the successful identification of S. enterica in complex food matrices (whole egg powder and beef samples) with satisfactory recovery rates. This BLI-based method holds great promise in serving as a novel diagnostic tool in the field of food safety and other related fields for the rapid, label-free, specific, and real-time detection of S. enterica.

中文翻译：

---

使用生物层干涉法快速无标记检测肠道沙门氏菌

沙门氏菌是一种重要的食源性病原体。它在食品中的快速可靠检测对于有效预防食源性疾病的爆发至关重要。在这里，我们为快速，特异性，无标记的，并实时检测的报告的新方法沙门氏菌使用抗体作为该受体与生物层干涉测量法（BLI）。在结合时间为 300 秒时，缓冲液中肠球菌的检测限为 1.6 × 10 ⁵ CFU/ml。该方法成功鉴定了S. enterica在复杂的食品基质（全蛋粉和牛肉样品）中具有令人满意的回收率。这种基于 BLI 的方法在作为食品安全领域和其他相关领域快速、无标记、特异性和实时检测肠球菌的新型诊断工具方面具有很大的前景。