Ammonia from livestock manure acts as a precursor to produce particulate matter (PM) by reacting with atmospheric chemical components volatilized from various sources. Ammonia itself acts as a toxic substance to human health, and thus has direct or indirect adverse effects on human health. This study aimed to verify the effectiveness and mechanism of action of Saccharomyces boulardii (SB) in reducing the ammonia emission from livestock manure. The specific ability of SB was confirmed through comparative verification with S. cerevisiae (SC) belonging to the same genus. SB and SC could use 50% of ammonia–nitrogen as inorganic nitrogen source in minimal medium. In the control group, the pH level of manure was significantly increased compared to the pH level at 0 h, and the DNA concentration of Proteus mirabilis, which increase the manure pH through ammonia production, was found to increase by 2.7-fold. Significant decrease in pH and proliferation of P. mirabilis was found in SB group compared to control (p < 0.05). The SB group also reduced the amount of ammonia emitted from manure by 25% for 35 days. These results suggested that SB contributed to reducing ammonia emission from manure by reducing pH and inhibiting HAB as well as removing ammonia–nitrogen. Accordingly, SB as a microbiological agent is expected to contribute not only to reduce ammonia emission but also to improve manure quality as a fertilizer.

中文翻译：

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啤酒酵母用于减少畜禽粪便氨气排放的特性

牲畜粪便中的氨通过与各种来源挥发的大气化学成分反应，作为产生颗粒物（PM）的前体。氨本身是对人体健康的有毒物质，因此对人体健康具有直接或间接的不利影响。这项研究旨在验证Saccharomyces boulardii（SB）减少牲畜粪便中氨气排放的有效性和作用机理。通过与属于同一属的酿酒酵母（SC）的比较验证，确认了SB的特异性能力。在基本培养基中，SB和SC可以使用50％的氨氮作为无机氮源。在对照组中，粪便的pH值与0 h时的pH值相比显着增加，而奇异变形杆菌的DNA浓度，通过产生氨提高粪便pH值的肥料发现增加了2.7倍。与对照组相比，SB组的pH值和P. mirabilis增殖明显降低（p <0.05）。SB组还连续35天将粪便中的氨气排放量减少了25％。这些结果表明，SB可以通过降低pH值，抑制HAB以及去除氨氮来减少粪肥中的氨气排放。因此，期望SB作为微生物剂不仅有助于减少氨的排放，而且还有助于提高肥料的肥料品质。SB组还连续35天将粪便中的氨气排放量减少了25％。这些结果表明，SB可以通过降低pH值，抑制HAB以及去除氨氮来减少粪肥中的氨气排放。因此，期望SB作为微生物剂不仅有助于减少氨的排放，而且还有助于提高肥料的肥料品质。SB组还连续35天将粪便中的氨气排放量减少了25％。这些结果表明，SB可以通过降低pH值，抑制HAB以及去除氨氮来减少粪肥中的氨气排放。因此，期望SB作为微生物剂不仅有助于减少氨的排放，而且还有助于提高肥料的肥料品质。