It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses. In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants, CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment. This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

中文翻译：

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转基因拟南芥中锦鸡儿 CkWRKY33 对干旱胁迫的耐受性增强

众所周知，WRKY转录因子在植物生长发育、防御调控和胁迫反应中发挥着重要作用。在这项研究中，从 Caragana korshinskii 克隆了一个 WRKY 转录因子 WRKY33。序列结构分析表明它属于I组类型。烟草表皮细胞中的亚细胞定位实验表明细胞核中存在 CkWRKY33。此外，CkWRKY33 在拟南芥中过度表达。表型调查显示，与野生型植物相比，过表达 CkWRKY33 的转基因植物在干旱胁迫处理后具有更高的存活率，以及相对的可溶性糖、脯氨酸和过氧化物酶含量，但丙二醛含量较低。这表明 CkWRKY33 的过表达导致转基因拟南芥的干旱胁迫耐受性增强。因此，CkWRKY33 可能作为参与 Caragana korshinskii 干旱胁迫响应的正调节因子。